油菜茎基溃疡病菌与黑胫病菌菌丝的生长动态  

The Research of the Mycelia Growth Dynamics of Leptosphaeria maculans and L. biglobosa

作　　者：叶芸 张建坤[2] 王琴 徐雪 王振华 

机构地区：[1]湖北出入境检验检疫局,武汉430050 [2]武汉理工大学理学院,武汉430070

出　　处：《湖北农业科学》2016年第18期4708-4710,4770,共4页Hubei Agricultural Sciences

基　　金：国家质检总局科技计划项目(2015IK123)

摘　　要：比较了油菜茎基溃疡病菌（Leptosphaeria maculans）和油菜黑胫病菌（L.biglobosa）在不同培养温度和2种培养基上的菌丝生长动态。结果表明,在PDA和V8培养基平板上都生长良好,油菜茎基溃疡病菌培养温度在28℃时,油菜黑胫病菌培养温度在25℃或28℃时,在PDA平板上菌丝生长最好;油菜黑胫病菌与油菜茎基溃疡病菌菌落生长速度相比,在最适培养温度（25℃或28℃）和相同的培养时间（21 d）内,二者在PDA平板上的菌落生长速度分别为5~7 mm/d和2.0 mm/d,前者的菌落直径约90 mm,是后者的2倍。因此,在温度为25℃或28℃的条件下,PDA培养基最适合这2种病菌的生长。The mycelia growth between Leptosphaeria maculans and L. biglobosa in different temperature on two kinds of culture medium were compared,which grew well in PDA and V8 medium plate, mycelia grows the best of L. maculans at 28 ℃on PDA culture medium, the best of L. biglobosa at 25 ℃ and 28 ℃ on PDA culture medium was more suitable for the growth of the strain hyphae than the V8 medium. The colony extension rate of L. maculans and L. biglobosa was 5~7 mm / d and 2.0 mm / d in optimal temperature（25 ℃ or 28 ℃） and（21 d） on PDA culture medium. The former colony diameter was2 times of the latter, about 90 mm. As a result, when the temperature was 25 ℃ or 28 ℃,PDA culture medium was the best suitable for the growth of the two kinds of fungi.

关 键 词：油菜茎基溃疡病菌(Leptosphaeria maculans) 油菜黑胫病菌(L.biglobosa) 菌丝生长 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]