拟南芥rd29A启动子的克隆及植物表达载体构建  

Isolation of Arabidopsis rd29A Promoter and Construction of Its Plant Expression Vectors

作　　者：申丽婕 于月华[1] 刘娜[1] 梁承娟 汪晓东[1] 谷月好 崔雪[1] 张振清[1] 倪志勇[1] 

机构地区：[1]新疆农业大学农学院/新疆农业大学农业生物技术重点实验室,乌鲁木齐830052

出　　处：《湖北农业科学》2016年第18期4824-4826,共3页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31360264);新疆维吾尔自治区高校科研计划科学研究重点项目(XJEDU2014I015);新疆维吾尔自治区自然科学基金项目(2014211A025;2016D01A043);中国博士后科学基金项目(2015M582741);新疆维吾尔自治区优秀青年科技人才培养项目;新疆维吾尔自治区优秀博士后普通资助项目;新疆维吾尔自治区级大学生创新创业训练计划项目(201510758126)

摘　　要：以拟南芥(Arabidopsis thaliana)基因组DNA为材料,根据Gen Bank中拟南芥rd29A启动子序列设计引物,采用PCR克隆一段长度为951 bp的rd29A启动子序列。采用Plant CARE启动子在线预测工具分析表明,rd29A启动子序列具有TATA-box、CAAT-box基本的顺式作用元件和1个脱落酸应答元件(ABRE)和2个脱水应答调控元件(DRE)。将rd29A启动子序列亚克隆到pcambia3301载体的多克隆位点,构建了由rd29A启动子驱动的植物表达载体。Based on the sequence of rd29 A promoter region in Gen Bank,primers of promoter sequences were designed. A 951 bp fragment was cloned from the Arabidopsis genomic DNA by PCR method. Promoter sequence analyzed by the database of Plant CARE showed that the sequence contains basic elements TATA-box,CAAT-box and one ABA responsive element（ABRE）,two dehydration responsive element（DRE）. rd29 A promoter was cloned to multiple cloning sites in pcambia3301, the plant expression vector which driven by rd29 A promoter was constructed.

关 键 词：拟南芥(Arabidopsis thaliana) RD29A 启动子 植物表达载体 

分 类 号：Q78[生物学—分子生物学]