中黑盲蝽丝氨酸蛋白酶基因克隆与序列分析  

Cloning and Sequence Analysis of Serine Proteinase of Adelphocoris suturalis Jakovlev

作　　者：刘巧英[1] 李梦歌[1] 刘雪飞[1] 余昊[1] 王运兵[1] 

机构地区：[1]河南科技学院资源与环境学院,河南新乡453003

出　　处：《湖北农业科学》2016年第21期5659-5663,共5页Hubei Agricultural Sciences

基　　金：转基因生物新品种培育专项(2013ZX08011-002)

摘　　要：采用兼并引物和RACE的技术,在中黑盲蝽(Adelphocoris suturalis Jakovlev)中获得丝氨酸蛋白酶的全长c DNA序列,将该基因命名为ASJSP,c DNA全长为958 bp,开放阅读框为885 bp,推测编码295个氨基酸,预计分子量为31.20 k Da,等电点p I为9.38,预测分子式为C1 374H2 222N390O407S15,不稳定系数为31.94,总亲水性系数为0.114,为疏水性稳定蛋白质。序列分析表明ASJSP与其他盲蝽科昆虫已知丝氨酸蛋白酶的核苷酸序列一致性为55.70%。RT-PCR with degenerate primers and RACE were used to amplify partial c DNA fragments and full c DNA,one serine protease gene from Adelphocoris suturalis Jakovlev was identified, designated ASJSP. The full-length c DNAs of ASJSP is958 bp,this gene contains 885 bp open-reading frames（ORF） which encodes a putative 295 amino acid protein,with a predicted molecular mass of 31.20 k Da and isoeletric point（p I） of 9.38. Its predicted molecular formula is C1 374H2 222N390O407S15. Bioinformatical analysis showed that it is a instability index is 31.94 and the GRAVY 0.114,suggesting that it is a stable hydrophobic protein. Sequences analysis showed that ASJSP had 55.70% identity with complete serine protease genes from other Miridae insects.

关 键 词：中黑盲蝽(Adelphocoris suturalis Jakovlev) 丝氨酸蛋白酶 RT-PCR 序列分析 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]