不同剂量培哚普利对兔急性心肌梗死后循环血内皮祖细胞动员作用  

Effect of Different Doses of Perindopril on Endothelial Progenitor Cell Mobilization in Rabbit Model of Acute Myocardial Infarction

作　　者：谈红[1] 孟楠[2] 张夏晓 苏莉[3] 张国明[1] 李晓燕[1] 公雪[1] 陈英剑[4] 姜蕴珊 

机构地区：[1]济南军区总医院心内科,山东省济南市250000 [2]锦州医科大学济南军区总医院研究生培养基地 [3]济南军区总医院干部保健二科,山东省济南市250000 [4]济南军区总医院检验科,山东省济南市250000

出　　处：《中国循环杂志》2016年第11期1120-1125,共6页Chinese Circulation Journal

基　　金：济南军区总医院院长基金(2013MS05)

摘　　要：目的：研究不同剂量培哚普利对兔急性心肌梗死（AMI）后循环血中内皮祖细胞（EPCs）数量的影响,及循环血血浆中血管内皮生长因子（VEGF）、血管紧张素Ⅱ（AngⅡ）及血管紧张素1~7（Ang1~7）含量的变化。方法：选择清洁级（2.2±0.3）kg新西兰大白兔,开胸及部分结扎左前降支处理,将存活的实验用兔随机分为四组,A组为假手术组（n=7）;B组为AMI组（n=6）;C组为小剂量培哚普利组（n=6）;D组为大剂量培哚普利组（n=6）。分别于1、3、7、14、28天采血,用流式细胞仪计数循环血中内皮祖细胞（EPCs）的数量,酶联免疫吸附法（ELISA）检测循环血中VEGF、AngⅡ、Ang1~7的含量。结果：AMI后,四组外周循环血中EPCs数量、VEGF的含量随着时间的推移均逐渐增加,于第7天达到高峰,之后逐渐降低,D组上述趋势变化最明显,且28天时D组较B组、C组仍能维持在较高水平[EPCs：（0.066±0.005）% vs （0.059±0.005）%,（0.062±0.003）%;VEGF（114.692±9.599）pg/ml vs （84.058±4.608）pg/ml,（102.570±10.869）pg/ml],差异均有统计学意义（P均〈0.05）;四组AngⅡ的含量增多,于第3天达到高峰,之后逐渐下降,下降幅度以D组最为明显;Ang1~7在手术组第1天有明显下降趋势,之后逐渐增多,随着时间推移呈逐渐增多的趋势,以D组更为明显。结论 ：培哚普利可促进AMI兔外周循环血中EPCs的动员及VEGF、Ang1~7含量的增多,降低AngⅡ的水平,且应用大剂量培哚普利干预效果更明显。Objective： To study different doses of perindopril on circulating blood endothelial progenitor cells（EPCs） mobilization and the levels of vascular endothelial growth factor（VEGF）, angiotensin II（Ang II）, angiotensin 1-7（Ang1-7） in rabbit model of acute myocardial infarction（AMI）. Methods： AMI model was established in New Zealand white rabbits by partial ligation of left anterior descending artery, the mean body weight of animal was（2.2±0.3） kg. Surviving rabbits were divided into 4 groups：（1） Sham operation group, n=7,（2） AMI group, n=6,（3） AMI＋small dose of perindopril group, n=6 and（4） AMI＋large dose of perindopril group, n=6. Peripheral blood was taken at 1, 3, 7, 14, 28 days of AMI, blood levels of EPCs were examined by flow cytometry; VEGF, Ang II and Ang1-7 were measured by ELISA.Results： Within 4 weeks of AMI, blood levels of EPCs, VEGF were increasing in all 4 groups, they reached the peakat day-7 followed by gradually decreasing, the most obvious trend was in Group（4）. Compared with Groups（2）,（3）, at day-28 of AMI, Group（4） had the higher levels of EPCs（0.066±0.005） % vs（0.059±0.005） %,（0.062±0.003） % and VEGF（114.692±9.599） pg/ml vs（84.058±84.608） pg/ml,（102.570±10.869） pg/ml, all P0.05. Ang II level was elevated after AMI, its reached the peak at day-3 followed by gradually decreasing, the most obvious reduction was in Group（4）. Ang1-7 level presented obvious decreasing trend at day-1 followed by gradually increasing; in Groups（3）,（4）, it was initially reduced and as time went on, the trend had been increasing especially in Group（4）.Conclusion： Perindopril may promote EPCs mobilization, increase blood levels VEGF, Ang1-7 and decrease Ang II in rabbit model of AMI; such effects were more obvious in large dose of perindopril intervention.

关 键 词：心肌梗死 内皮祖细胞 培哚普利 

分 类 号：R541[医药卫生—心血管疾病]