广西烟草立枯病菌和靶斑病菌菌丝融合群初步分析  

Anastomosis groups of Rhizoctonia solani causing tobacco sore shin and target spot in Guangxi

作　　者：陈媛媛[1] 谭海文[2] 卢燕回[3] 黎起秦[1] 林纬[1] 袁高庆[1] 

机构地区：[1]广西大学农学院,广西南宁530005 [2]田阳县农业局,广西田阳533600 [3]中国烟草总公司广西壮族自治区公司,广西南宁530022

出　　处：《广东农业科学》2016年第10期106-111,F0002,共7页Guangdong Agricultural Sciences

基　　金：中国烟草总公司广西壮族自治区公司科技创新项目(2014-07)

摘　　要：为了明确广西烟草立枯病菌和靶斑病菌的菌丝融合群,从广西发病烟草植株上分离了2株烟草立枯病菌菌株和3株靶斑病菌菌株。经形态特征和细胞核染色观察发现,这5株菌株均属于多核立枯丝核菌。依据供试菌株与标准菌株的菌丝融合反应,发现广西烟草立枯病菌和靶斑病菌均包含AG-2和AG-4两个菌丝融合群。进一步分析5个菌株的r DNA-ITS序列,初步明确这2种病原菌包括AG-4 HG-I和AG-2-2 IIIB 两个菌丝融合群,与菌丝融合反应分析结果相吻合。首次在国内从烟草靶斑病组织上分离到AG-2 和AG-4 融合群。In order to investigate the anastomosis groups of the pathogens causing tobacco sore shin and target spot in Guangxi,two strains were isolated from diseased basal stems and three strains were isolated from diseased leaves.Based on morphological characteristics and nuclear staining,five strains were identified as Rhizoctonia solani and they all belonged to multinucleate.Through hyphal anastomosis reactions between tested strains and standard strains,the pathogens of tobacco sore shin and target spot in Guangxi were all divided into two anastomotic groups： AG-2 and AG-4.Moreover,ITS sequences analysis of the five strains indicated that the two anastomosis groups were AG-4 HG-I and AG-2-2 IIIB,and it was in accord with the result of hyphal anastomosis reaction.AG-2 and AG-4 were firstly found in tobacco leaf in China.

关 键 词：烟草立枯病 烟草靶斑病 立枯丝核菌 菌丝融合群 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]