淫羊藿苷对ox-LDL诱导巨噬细胞凋亡的作用及其机制的研究  

Effects of icariin on macrophage apoptosis induced by ox-LDL and the underlying mechanisms

作　　者：罗娟[1,2] 戢艳琼[3] 谭艳[1] 潘龙瑞[1] 汪选斌[2] 李洪亮[2] 张秋芳[1,2] 

机构地区：[1]湖北医药学院武当特色中药湖北省重点实验室,十堰442000 [2]湖北医药学院药理教研室,十堰442000 [3]湖北十堰市太和医院心血管内科,十堰442000

出　　处：《中国新药杂志》2016年第23期2701-2706,共6页Chinese Journal of New Drugs

基　　金：国家自然科学基金资助项目(81303254);湖北省教育厅重点项目(D20122402);湖北医药学院项目(2010QDJ12;2012GPY11);武当特色中药研究湖北省重点实验室资助项目(WDCM003)

摘　　要：目的:研究淫羊藿苷(icariin,ICA)对氧化低密度脂蛋白(oxidized low density lipoprotein,oxLDL)诱导的巨噬细胞凋亡的影响,并探讨其机制。方法:体外培养巨噬细胞RAW264.7,给予ICA(10,20,40μmol·L^(-1))或5 mmol·L^(-1)4-苯丁酸(4-phenylbutyric acid,4-PBA)预处理1 h,然后加入100 mg·L-1ox LDL继续培养24 h,采用MTT法检测细胞活力;油红O染色检测泡沫细胞的形成;Annexin V-FITC/PI和比色法检测细胞凋亡和caspase-3活性,Western blot检测GRP78的表达水平。结果:与ox LDL组相比,ICA预处理组减轻ox LDL诱导的巨噬细胞的损伤,表现为细胞活力的增加,凋亡率下降,caspase-3活性降低,与内质网抑制剂4-PBA相似,降低内质网应激标志蛋白GRP78表达。结论:ICA可能通过抑制ox LDL诱导的过强内质网应激,降低caspase-3活性,抑制ox LDL诱导的细胞凋亡。Objective： To investigate the inhibitory effects of icariin（ ICA） on oxidized low density lipoprotein（ ox LDL）-induced macrophage apoptosis and the underlying mechanisms. Methods： RAW264. 7macrophages were pretreated with ICA（ 10,20,40 μmol·L-1） or 5 mmol·L-14-phenylbutyric acid（ 4-PBA） for 1h,then treated with ox LDL（ 100 mg·L-1） for 24 h. The cell viability and apoptosis rate were detected by MTT assay and annexin-FITC / PI double staining method,respectively. Intracellular lipid droplets were assayed by oil red O staining,caspase-3 activity was measured by chromatometry,and endoplasmic reticulum stress markers（ GRP78） was detected by Western blot. Results： Pretreatment with ICA（ 20,40 μmol·L-1） could protect macrophages from injury induced by ox LDL,as shown by increased cell viability and decreased apoptotic rate and significantly attenuated lipid accumulation in RAW264. 7 cells. Compared with ox LDL group,the activity of capase-3 and the protein level of GRP78 in the macrophages were decreased in ICA pretreatment group. Conculsion：ICA can prevent macrophages damage induced by ox LDL,and the mechanisms may be partially involved with itsability to decrease the activity of caspase-3 and down-regulate endoplasmic reticulum stress.

关 键 词：淫羊藿苷 巨噬细胞 氧化低密度脂蛋白 内质网应激 

分 类 号：R392[医药卫生—免疫学] R96[医药卫生—基础医学]