电针大肠俞募穴对功能性便秘小鼠结肠内GDNFmRNA、RaimRNA表达影响的研究  

Effects of Electroacupuncture Back-shu and Front-mu Points on the GDNF-mRNA,RaimRNA Expression in Colonic Tissne for Mice of Functional Constipation

作　　者：张微[1] 李瑛[1] 刘丽莎[1] 罗芳丽[1] 赵中亭[1] 

机构地区：[1]成都中医药大学,成都610072

出　　处：《中国中医基础医学杂志》2016年第11期1522-1524,1551,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金面上项目(81273853)-针刺俞募穴治疗功能性便秘的EGC及ENS-ICC-SMC功能网络调整机制研究

摘　　要：目的:探讨电针大肠俞募穴对功能性便秘小鼠结肠组织内GDNFmRNA、Rai mRNA表达的影响。方法:48只小鼠按随机数字表法分为对照组、模型组和电针组。采用复方地芬诺酯灌胃造模,电针刺激"天枢"、"大肠俞"后观察结肠上皮细胞形态、结肠组织内GDNFmRNA、Rai mRNA的表达情况。结果:模型组小鼠结肠上皮细胞凋亡增多,GDNFmRNA、RaimRNA表达降低。电针组结肠上皮细胞凋亡减少,GDNFmRNA、RaimRNA表达增强。结论:电针能改善功能性便秘小鼠结肠上皮细胞凋亡情况,上调GDNFmRNA、Rai mRNA的表达水平。Objective To explore the mechanism of electroaeupuncture （ EA ） Back-shu and Front-mu points on GDNFmRNA ,Rai mRNA in colon in functional constipation （FC） mice. Methods： 48 mice were randomly divided into control group, model group, EA group. Using compound diphenoxylate orally modeling method and EA ＂ Tianshu＂, ＂Daehangshu＂ , the morphology of colonic epithelial structure, the level of GDNFmRNA, RaimRNA in colon were observed by TUNELand in situ hybridization. Results The expression of GDNF mRNA,, RaimRNA in model group was significantly decreased with more damage depithelial cells. EA back-Shu and front-Mu points can improve the expression of GDNF mRNA, RaimRNA. TUNEL found that acupuncture can repair damage depithelial cells. Conclusion ： EA hack-Shu and front- Mu points can up-regulate the expression of GDNF mRNA,RaimRNA in FC mice.

关 键 词：电针 俞募穴 功能性便秘 GDNFmRNA RaimRNA 

分 类 号：R245.97[医药卫生—针灸推拿学]