消痰通腑方对结直肠癌前病变模型小鼠结直肠黏膜上皮NF-κB/IL-6/STAT3和AICD蛋白表达水平的影响  

Effects of Xiaotan Tongfu Decoction on protein expression of NF-κB/IL-6/STAT3 and AICD in colorectal mucosal epithelium of mice with colorectal precancerous lesions model

作　　者：刘煊[1] 顾雨芳[1] 修丽娟[1] 孙昱玮 矫健鹏[1] 赵婧[1] 余嘉惠 岳小强[1] 

机构地区：[1]第二军医大学附属长征医院中医科,上海200003

出　　处：《上海中医药杂志》2016年第12期70-75,共6页Shanghai Journal of Traditional Chinese Medicine

基　　金：军队中医药科研专项课题基金资助项目(2010z131);上海市名老中医学术经验研究工作室建设项目(ZYSNXD-CC-MZY054);上海市长征医院青年启动基金资助项目(2015CZQN11)

摘　　要：目的探讨消痰通腑方对结直肠癌前病变模型小鼠结直肠黏膜上皮核转录因子-κB(NF-κB)/白介素-6(IL-6)/信号传导与转录激活因子3(STAT3)和活化诱导胞嘧啶核苷脱氨酶(AICD)蛋白表达水平的影响。方法 C57BL/6小鼠90只,按随机数字表法分为空白组、模型组、西药组(柳氮磺胺吡啶组)、中药组(消痰通腑方组)和中西医结合组(柳氮磺胺吡啶加消痰通腑方组)。除空白组外,其余四组采用AOM/DSS"四步三循环法"造模。观察各组小鼠一般状况及结直肠黏膜上皮病理情况,计算结直肠癌前病变成瘤率,Western blotting法检测造模过程中(6周、14周、18周)小鼠结直肠黏膜上皮中NF-κB/IL-6/STAT3及AICD的蛋白表达水平。结果运用消痰通腑方后,小鼠一般状况较模型组好。中西医结合组的成瘤率(33.33%)低于模型组成瘤率(50.00%)。在14周和18周,与西药组比较,中药组和中西医结合组的NF-κB表达水平变化不显著,而IL-6、STAT3和AICD表达水平均下调;与中药组比较,中西医结合组在IL-6和STAT3表达上差异不明显,但AICD表达显著低于中药组。结论消痰通腑方能够改善小鼠一般状况,降低结直肠癌前病变的发生率,下调结直肠癌前病变小鼠直肠黏膜上皮中IL-6/STAT3蛋白的表达水平,下调AICD蛋白表达水平,此作用途径可能是其降低结直肠癌前病变发生率、预防结直肠癌发生的机制之一。Objective To discuss the effects of Xiaotan Tongfu（ XTTF） Decoction on protein expression of nuclear factor-κB（ NF-κB） / interleukin-6（ IL-6） /signal transducer and activator of transcription 3（ STAT3） and activation induced cytidine deaminase（ AICD） in colorectal mucosal epithelium of mice with colorectal precancerous lesions model. Methods According to the random number table,ninety C57 BL /6 mice were randomly divided into five groups： the control group,model group,western medicine group（ Sulfasalazine group）,traditional Chinese medicine group（ XTTF Decoction group） and the combination group（ Sulfasalazine and XTTF Decoction group）. Except the control group,model was established in the other four groups by the AOM / DSS＂four-step three-cycle method＂. General condition of mice and pathological changes of colorectal mucosa epithelium in each group were observed. The rate of tumor formation of colorectal precancerous lesions was calculated. The protein expression of NF-κB / IL-6 / STAT3 and AICD in colorectal mucosal epithelium of mice during the modeling process（ 6 weeks,14 weeks,18 weeks） were detected by Western Blotting. Results After the treatment of XTTF Decoction,the general condition of mice were better than the mice in the model group. The rate of tumor formation in the combination group（ 33. 33%） was lower than that in the model group（ 50. 00%）. After treatment for 14 and 18 weeks,compared with the western medicine group,the changes of NF-κB expression in the traditional Chinese medicine group and combination group were not significant,but the expression levels of IL-6,STAT3 and AICD were all significantly down-regulated. Compared with the traditional Chinese medicine group,the differences on the expression of IL-6 and STAT3 in the combination group were not significant,but the AICD expression was significantly lower than that in the traditional Chinese medicine group. Conclusion XTTF decoction can improve the general condition of mice with colorecta

关 键 词：结直肠癌前病变 核转录因子-κB 白介素-6 信号传导与转录激活因子3 活化诱导胞嘧啶核苷脱氨酶 消痰通腑方 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]