多指标综合评分法优选意大利牛舌草总黄酮的提取工艺  

Optimization of extraction process of total flavonoids from Anchusa italica Retz. by multi-index comprehensive score method

作　　者：路文杰[1] 程雪梅[2] 滕亮[3,4] 马桂芝[1] 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011 [2]上海中医药大学中药研究所,中药标准化教育部重点实验室,中药新资源与质量标准综合评价国家中医药管理局重点研究室,上海中药标准化研究中心,上海201210 [3]新疆医科大学第一附属医院药学部,新疆乌鲁木齐830011 [4]新疆医科大学第一附属医院昌吉分院,新疆昌吉831100

出　　处：《上海中医药杂志》2016年第12期89-93,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：新疆维吾尔自治区科技计划资助项目(20113015-2)

摘　　要：目的优选意大利牛舌草总黄酮的提取工艺。方法以总黄酮提取率、芦丁提取率及出膏率为考察指标,采用两种多指标综合评分法及L9(34)重复正交试验设计,优选意大利牛舌草回流提取法的工艺条件。结果用于测定提取物中总黄酮含量与芦丁含量的测定方法均符合方法学要求。意大利牛舌草总黄酮回流提取法优选工艺条件为:料液比1∶20;提取温度100℃;乙醇体积分数75%;提取2次,每次2 h。结论验证试验表明优选出的提取工艺简便、合理、可行,为后续研究奠定了基础。Objective To optimize the extraction process of total flavonoids from Anchusa italica Retz.. Methods According to the extraction efficiency of total flavonoids and rutin and the extraction ratio,the process of refluxing extraction of Anchusa italica Retz. was optimized by two kinds of multi-index comprehensive score method and L9（ 3^4） repeated orthogonal design. Results The methods of content determination for total flavonoids and rutin in the extracts were accordance with the requirements of methodology. The optimized refluxing extraction process of total flavonoids from Anchusa italica Retz. as follows： the ratio of material and liquid is 1 ∶ 20（ w / v）,extraction temperature is 100 ℃,the volume fraction of ethanol is 75%,twice extraction and each 2 hours. Conclusion The validation test shows that the optimized extraction process is simple,reasonable and feasible,which provides the theory basis for the subsequent studies.

关 键 词：意大利牛舌草 回流提取法 多指标综合评分 重复正交试验设计 

分 类 号：R284.2[医药卫生—中药学]