硫化氢对百草枯中毒大鼠急性肺损伤及炎症介质的影响  

Effects of hydrogen sulfide on acute lung injury and inflammatory factors in rats with paraquat poisoning

作　　者：舒维[1] 陈文龙[2] 谢姝[2] 汤旭惠[2] 肖翔宇[2] 傅强[2] 陈宁南[2] 陈运和[2] 

机构地区：[1]江西省胸科医院呼吸科,南昌330006 [2]江西省人民医院急诊科,南昌330006

出　　处：《江西医药》2016年第11期1162-1164,共3页Jiangxi Medical Journal

基　　金：江西省卫生计生委科技计划(20155006)

摘　　要：目的观察硫化氢(H_2S)对百草枯(PQ)中毒大鼠急性肺损伤(ALI)及炎症介质的影响。方法将30只雄性SD大鼠随机分为3组,每组10只,正常对照组(A组),PQ中毒组(B组),NaHS干预组(C组)。B、C组采用PQ灌胃(80mg/kg)复制大鼠PQ中毒肺损伤模型,B组同时腹腔注射生理盐水(6ml/kg),每日两次,C组PQ染毒后,同时腹腔注射NaHS(1.4umol/kg),每日两次;A组采用等量生理盐水灌胃,同时腹腔注射生理盐水(6ml/kg),每日两次。72h后取血检测肿瘤坏死因子-а(TNF-а)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)的含量;在光学显微镜下观察肺组织病理学改变,进行肺损伤病理评分。结果 B组中TNF-а、IL-6、及IL-8含量明显高于A组(P<0.01);C组中TNF-а、IL-6、及IL-8含量较B组明显减低(P<0.05);B组大鼠肺损伤病理评分高于A组(P<0.01);C组大鼠肺损伤病理评分较B组减低(P<0.01)。结论 H_2S具有抑制百草枯中毒大鼠炎症介质表达,减轻大鼠急性肺损伤作用。Objective To investigate the effect of hydrogen sulfide（H2S） on acute lung injury（ALI） and inflammatory factors in rats with paraquat poisoning. Methods 30 male SD rats were randomly divided into 3 groups,10 rats in each group,normal control group（A group）,PQ poisoning group（B group）,NaHS intervention group（C group）. B,C,group of rats were made into the model of lung injury by pouring PQ（80mg/Kg） into the stomach,B group was injected with normal saline（6ml/kg） at the same time,two times a day. C group rats were exposed to PQ,while intraperitoneal injection of NaHS（1.4μmol/kg）,two times a day. In group A,the same amount of normal saline was used to fill the stomach,normal saline（6ml/kg） was injected by intraperitoneal at the same time,two times a day. Determinating the concentration of tumor necrosis factor-а（TNF-а）,interleukin-6（IL-6）,interleukin-8（IL-8） in peripheral blood after 72 hours. Observing the pathological changes of lung tissue,counting lung injury score under optical microscope. Results In group B,TNF-а,IL-6,and IL-8 were significantly higher than that of A group（P〈0.01）. In group C,TNF-а,IL-6,and IL-8 was significantly lower than that of group B（P〈0.05）. The lung injury score in B group was significantly higher than that in A group（P 〈0.01）. The lung injury score in the C group was significantly lower than that in the B group（P〈0.01）. Conclusion H2S can inhibit the expression of inflammatory mediators in rats with paraquat poisoning,and reduce the acute lung injury in rats.

关 键 词：硫化氢 百草枯 急性肺损伤 

分 类 号：R595.4[医药卫生—内科学]