毛细管电泳-电化学检测法测定淮山中薯蓣皂苷和腺苷含量  

Determination of Dioscin and Adenosine in Yam by Capillary Electrophoresis with Electrochemical Detection

作　　者：胡月芳[1] 黄志强[1] 李金芳[1] 

机构地区：[1]贺州学院化学与生物工程学院,广西贺州542899

出　　处：《分析科学学报》2016年第6期795-799,共5页Journal of Analytical Science

基　　金：广西高校科学技术研究项目(No.2013YB238);贺州市科学研究与技术开发计划(贺科转1408035)

摘　　要：建立了毛细管电泳-电化学检测（CE-ED）法测定淮山中薯蓣皂苷和腺苷的含量。考察了检测电位、运行缓冲液浓度和pH、分离电压及进样时间等的影响。在优化的实验条件下,对0.1mg/L薯蓣皂苷、腺苷在9min内实现了分离,其线性范围分别为0.1~1 000μg/L和0.1~1 200μg/L;检出限分别为0.04μg/L和0.03μg/L,峰电流的相对标准偏差（RSD,n=6）分别为1.7%、1.5%,迁移时间的RSD分别为0.7%、0.6%。该方法已用于淮山样品中薯蓣皂苷和腺苷的测定,样品加标回收率在98.0%~102.5%之间,RSD≤2.3%。A method of capillary electrophoresis coupled with electrochemical detection（CE-ED） has been established for the determination of dioscin and adenosine in yam. The effects of detection potential, concentration and pH of running buffer, separation voltage and injection time on the detection were investigated. Under the optimum conditions, 0. 1 mg/L dioscin and adenosine mixed standard solution were detected for six times continuous injection, and good baseline separation and highly sensitive detection were achieved in 9 minutes. A good linear relationship was obtained between peak currents and dioscin and adenosine concentration ranging from 0.1-1 000 ffg/L and 0. 1-1 200 μg/L, with the detection limits （S/N= 3） of 0.04 fig/L, 0. 03 μg/L, respectively. The relative standard deviations（RSDs） of peak currents of dioscin and adenosine were 1.7%, 1.5 %, the RSD of the migration time were 0. 7%, 0.6%, respectively. The method was successfully applied to the analysis of dioscin and adenosine in samples of yam. The spiked recoveries were in the range of 98.0%-102.5%, with RSDs less than 2.3%,

关 键 词：毛细管电泳-电化学检测 淮山 薯蓣皂苷 腺苷 

分 类 号：O657.8[理学—分析化学]