蛋白质印迹模板的制备及在尿液蛋白测定中的应用  

Molecular Imprinted Materials for Determination of Protein in Human Urine

作　　者：邢建华[1] 薄惠[1] 张强[1] 

机构地区：[1]黄河科技学院医学院,河南郑州450063

出　　处：《分析科学学报》2016年第6期869-872,共4页Journal of Analytical Science

基　　金：国家自然科学基金(No.U1204214)

摘　　要：以牛血清蛋白（BSA）为分子模板,甲基丙烯酸（MAA）为功能单体,乙二醇二甲基丙烯酸酯（EDGMA）为交联剂,偶氮二异丁腈（AIBN）为引发剂,制备了BSA分子印迹模板,研究了该模板对BSA的吸附性能,并以其作为净化材料去除人尿中的干扰物质,测定了人尿中的蛋白质。结果表明：蛋白质模板吸附和洗脱的最佳pH值分别为5.3和5.6,最佳吸附时间为2h,最大吸附容量为72mg/g。三种人尿样品中蛋白质的含量分别为35.4、18.9、24.2mg/L,回收率为92.4%~103.7%。该方法可用于人尿中蛋白质含量的测定。The protein imprinted polymer is synthesized with bovine serum albumin（BSA） as template, methacrylic acid （MAA） as functional monomer, ethylene glycol dimethacrylate （EGDMA） as a crosslinker, and azobisisobutyronitrile as initiator. Afterwards, the protein imprinted material was used as purification materials for determination of protein in human urine. The results show that the best absorption and clean pH value is 5. 3 and 5. 6, respectively, and the adsorption capacity is 72 mg/L. Moreover,the concentration of protein in three human urine samples were determined to be 35.4,18.9 and 24. 2 mg/L, with the recovery between 92. 4% and 103. 7%. The method was suitable for the determination of protein in human urine.

关 键 词：分子印迹技术 人尿蛋白 吸附 

分 类 号：O658[理学—分析化学]