康力欣胶囊对胃癌细胞株细胞周期和细胞凋亡的影响  

Effect of Kanglixin Capsules on Cell Cycle and Apoptosis of Gastric Cancer Cell Line

作　　者：刘学芳[1] 董浩然[1] 张娜[2] 冯素香[1] 

机构地区：[1]河南中医药大学中医内科学重点学科开放实验室,河南郑州450046 [2]河南中医药大学基础医学院人体解剖与组织胚胎学学科,河南郑州450046

出　　处：《云南中医学院学报》2016年第6期10-13,20,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：河南省教育厅科学技术研究重点项目(14B310011)

摘　　要：目的研究抗肿瘤药康力欣胶囊对胃腺癌细胞株SGC-7901细胞增殖、细胞周期和细胞凋亡的影响。方法以康力欣胶囊2,1,0.1mg·mL^(-1)接种于细胞12,24,36,48h后,采用四甲基偶氮唑盐(MTT)法测定细胞抑制率,采用流式细胞仪检测细胞周期各时相比率的变化,荧光显微镜观察法检测各组细胞凋亡的形态变化。结果 MTT检查结果显示,康力欣胶囊抑制率呈明显的时间-剂量-效应关系;细胞周期检测结果显示,康力欣胶囊作用SGC-7901细胞后将细胞周期阻滞在G1期,同时下调S期和G2期细胞比例。荧光显微镜观察结果显示,康力欣胶囊组SGC-7901出现大量激发绿色荧光的早期凋亡细胞和同时激发红色荧光的晚期凋亡细胞。结论康力欣胶囊对SGC-7901的抑制作用可以通过阻滞细胞周期和诱导细胞凋亡来实现。Objective To observe the effects of the antitumor medicine-Kanglixin capsule on cell proliferation, cell cycle and apoptosis of the SGC-7901 cell. Methods After inoculated for 12, 24, 36, and 48 hours respectively, the action of Kanglixin capsule on growth inhibiting rate was detected by MTF, the DNA content of cell cycle in each phase was detected with flow cytometry and the morphologie change of cell apoptosis was observed through fluorescence microscope. Results The result of MTT method reveals that the inhibition rate of Kang Lixin capsule has a close time -dose-effect relationship;the flow cytometry result indicates that Kanglixin capsule can block cell cycle in G1 phase, meanwhile it decreases the percntagc of cells in S phase and G2 phase;the fluorescence microscope observes that Kanglixin capsule could induce early apoptosis and late apoptosis of SGC-7901 cells. Conclusion The inhibition of Kanglixin capsule on SGC-7901 cell can be realized by means of blocking cell cycle and inducing the cell apoptosis.

关 键 词：康力欣胶囊 胃癌细胞株 体外抑制 细胞周期 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]