高效液相色谱-蒸发光散射检测法同时测定壮药愈疡散中3种主要活性成分的含量  

Simultaneous determination of three main active ingredients in Zhuang medicine Yuyangsan by HPLC-ELSD

作　　者：黄志飘 覃洁萍[1] 刘鹏飞[1] 奉艳花 王淼[1] 常敬芳 刁宇[1] 

机构地区：[1]广西中医药大学药学院,广西南宁530001

出　　处：《中国医院药学杂志》2016年第23期2062-2065,共4页Chinese Journal of Hospital Pharmacy

基　　金：广西卫生厅中医药科技专项课题(编号:GZYZ1108);广西高校科技创新能力提升工程专项(编号:J15184)

摘　　要：目的:建立测定壮药愈疡散中黄芪甲苷、紫丁香苷与长梗冬青苷含量的方法。方法:采用高效液相色谱-蒸发光散射检测(HPLC-ELSD)法,色谱柱为Shim-pack CLC-ODS(150 mm×4.6 mm,5μm),以乙腈-水为流动相,梯度洗脱,流速1 mL·min^(-1),柱温20℃;检测参数为漂移管温度为90℃,雾化器温度为45℃,气体流量为1.60 L·min^(-1),增益值为1.0。结果:黄芪甲苷、紫丁香苷和长梗冬青苷分别在0.46~2.76μg(r=0.999 2)、0.70~4.20μg(r=0.996 2)和1.06~6.36μg(r=0.998 6)范围内,其峰面积的自然对数与进样量的自然对数呈良好的线性关系;平均加样回收率分别为95.89%、95.49%和97.66%,RSD为2.79%、3.96%和3.60%(n=6)。结论:该法简便快速、重复性好,结果准确,可作为壮药愈疡散的质量控制方法。OBJECTIVE To establish a method for content determination of astragalosideⅣ,syringin and pedunculoside in Zhuang medicine Yuyangsan.METHODS HPLC-ELSD method was used.Analysis was carried on Shim-pack CLC-ODS（150mm×4.6 mm,5μm）column with acetonitrile-water system as mobile phase in gradient elution.The flow rate was 1 mL·min-（-1）,column temperature was 20 ℃.Drift tube temperature was 90 ℃,atomizing air temperature was 45 ℃.Gas flow rate was 1.60 L·min-（-1） and gain value was 1.RESULTS Linear ranges of astragalosideⅣ,syringin and pedunculoside were 0.46-2.76μg（r=0.999 2）,0.70-4.20μg（r=0.9962）and 1.06-6.36μg（r=0.998 6）,respectively.Average recoveries were95.89%（RSD=2.79%）,95.49%（RSD=3.96%）and 97.66%（RSD=3.60%）.CONCLUSION This method is simple,rapid and steady,with good repeatability,and can be used for quality control for Zhuang medicine Yuyangsan.

关 键 词：黄芪甲苷 紫丁香苷 长梗冬青苷 含量测定 高效液相色谱-蒸发光散射检测 壮药愈疡散 

分 类 号：R917[医药卫生—药物分析学]