二陈汤加味对慢性阻塞性肺疾病氧化应激、沉默信息调节因子1表达的影响  

Effect of supplemental Erchen decoction on role of oxidative stress and Sirt1 in rats with chronic obstructive pulmonary disease

作　　者：陈四清[1] 谢文英[1] 尚立芝[1] 季书 潘晓丽[1] 刘坦[1] 胡文豪[1] 

机构地区：[1]河南中医药大学基础医学院,河南郑州450046

出　　处：《中国老年学杂志》2016年第23期5774-5777,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金面上项目(81573881);郑州市科技领军人才项目(121PLJRC535);河南省高等学校重点科研项目(15A360030);河南省科支重点攻关(152102310337);河南省教育厅资助项目(2010A360024);河南省科技攻关项目(112102310314);河南中医学院研究生教育研究课题(2014YJX001);河南中医学院基础医学院教学项目(2015JCJX10);河南中医学院大学生创新学习项目(4XM〔2015〕0017;CXXM〔2015〕0063);河南省中医药科学研究专项课题(2013ZY02070)

摘　　要：目的探讨二陈汤加味对慢性阻塞性肺疾病(COPD)大鼠氧化应激及沉默信息调节因子(Sirt)1表达的影响。方法 50只SD大鼠随机分为正常组、模型组、二陈汤加味20、10、5 g/kg剂量组,10只/组。香烟烟熏加脂多糖制备COPD动物模型,造模30 d。第31天起,对正常组、模型组灌胃(ig)生理盐水10 ml/kg,3个二陈汤加味组分别以20、10、5 g/kg剂量ig,连续14 d。检测肺功能、血清谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性,丙二醛(MDA)含量;酶联免疫法(ELISA)测定外周血单个核细胞中Sirt1浓度,酶联免疫荧光法测定外周血单个核细胞中Sirt1活性,实时荧光定量PCR检测外周血单个核细胞中Sirt1 mRNA表达,光镜观察炎细胞及肺组织病理形态。结果与正常组比较,模型组肺匀浆中SOD和GSH-Px活性均显著降低(均P<0.01),MDA含量显著增加(P<0.05),支气管肺泡灌洗液(BALF)中炎细胞总数和中性粒细胞数均显著增高(均P<0.01),模型组外周血单个核细胞中Sirt1 mRNA表达,Sirt1含量及其活性均显著减低(P<0.05或P<0.01)。与模型组比较,二陈汤加味10、20 g/kg剂量组BALF中炎细胞计数、中性粒细胞和巨噬细胞数显著减少(P<0.05或P<0.01);GSH-Px和SOD活性均显著增高,MDA含量显著降低(P<0.05或P<0.01),二陈汤加味10、20 g/kg剂量组外周血单个核细胞中Sirt1 mRNA表达,Sirt1含量及其活性均显著升高(P<0.05或P<0.01)。结论二陈汤加味有抗氧化损伤作用,其可能通过增加SOD和GSH-Px活性,减少MDA生成,增强Sirt1基因的表达,提高Sirt1活性,发挥抗氧化损伤而保护肺的结构与功能。Objective To study the effect of supplemental Erchen decoction on Sirt1 levels in serum,the activity of Sirt1 in rats with chronic obstructive pulmonary disease（ COPD）.Methods COPD was made by smoke combined with LPS. Rats were randomly divided into normal,model,Supplemental Erchen decoction groups（ 5,10,20 g/kg）. Enzyme-linked immunosorbent assay was used to determine serum Sirt1 level in rat. The activity of Sirt1 was determined by enzyme-linked immune fluorescence. Real-time quantitative PCR method was used to detect the expression of Sirt1 mRNA.Results The levels of SOD and GSH-Px in lung tissue were significantly lower and MDA was higher in model group than those of normal group（ P〈0.01）. The total number of neutrophils and inflammatory cells were significantly increased in model group than those of normal group in bronchoalveolar lavage fluid（ BALF）（ P〈0.01）. mRNA,content and activity of Sirt1 in model group were higher than those in normal group significantly（ P〈0.01）. Compared with model group,the inflammatory cells counts,neutrophils,macrophages were decreased（ P〈0. 01,P〈0. 05）,SOD,GSH-Px activity were increased,MDA content was decreased（ P〈0. 01,P〈0.05）,mRNA,content and activity of Sirt1 in 10,20 g/kg Supplemental Erchen decoction groups. Conclusions Supplemental Erchen decoction has anti-oxidative effect,which may be related with increasing SOD,GSH-Px activities,reducing MDA,increasing expression of Sirt1 gene and enhancing activity of Sirt1 so that protecting lung structure and function by anti-oxidative injury.

关 键 词：慢性阻塞性肺疾病 支气管肺泡灌洗液 氧化应激 沉默信息调节因子1 

分 类 号：R285.5[医药卫生—中药学]