膳食脂肪含量的改变对糖尿病大鼠胃促生长素水平的影响  

Effect of changes in dietary fat content on the ghrelin levels in diabetic rats

作　　者：陈云霞 高倩 刘春燕 赵淑玲 

机构地区：[1]河北省沧州市人民医院内分泌科,河北沧州061000

出　　处：《河北医科大学学报》2016年第12期1379-1382,共4页Journal of Hebei Medical University

摘　　要：目的观察糖尿病大鼠膳食脂肪含量变化对体质量、不同部位脂肪含量及胃促生长素的影响。方法将60只健康雄性SD大鼠,分为正常对照组15只,余45只大鼠给予高脂饲料喂养8周,联合小剂量链脲佐菌素腹腔注射建立糖尿病模型,造模成功36只。采用随机数字表法将造模成功的36只大鼠分为普通饮食糖尿病组和高脂饮食糖尿病组各18只。正常对照组和普通饲料糖尿病组给予普通饲料,高脂饮食糖尿病组给予高脂饲料,喂养6周后处死大鼠,收集血液和脂肪组织,采用酶联免疫吸附测定方法检测血浆胃促生长素水平。结果普通饮食糖尿病组和高脂饮食糖尿病组大鼠体质量、附睾的脂肪、肾周脂肪、肠系膜脂肪均高于正常对照组,胃促生长素均低于正常对照组。结论膳食脂肪含量的变化对血浆胃促生长素水平有调节作用,能影响体质量及能量摄入。Objective To study the regulation of dietary fat in diabetic rats on the weight,different parts of the fat and ghrelin.Methods Sixty male SD rats were divided into two groups randomly：15normal control rats and 45 test rats.The test rats were fed with high fat diet for eight weeks and a single intraperitoneal injection of streptozotocin（STZ,30mg/kg）to establish an animal model of diabetes.36 diabetic rat model was successfully established.The diabetic rats were randomly divided into following groups：18general diet diabetic rats and 18high-fat diet diabetic rats.The normal control rats and general diet diabetic rats were fed with general diet.The high-fat diet diabetic rats were fed with high-fat diet.After six weeks,all rats were killed.Blood and adipose tissue samples were collected in all rats.Serum ghrelin was detected by enzymelinked immunoassay.Results The general diet diabetic rats and high-fat diet diabetic rats were significantly higher in weight,epididymal fat,epinephrine fat and mesenteric fat than normal control rats,and were significantly lower in plasma ghrelin concentration than normal control rats.Conclusion Changes in dietary fat content play a regulatory role on levels of ghrelin in plasma that affect weight and energy intake.

关 键 词：糖尿病 膳食脂肪类 胃促生长素 大鼠 

分 类 号：R587.1[医药卫生—内分泌]