稳心颗粒对AngⅡ诱导H9C2细胞肥大模型骨架蛋白的影响  

The Influence of Wenxin Granule on the Cytoskeleton Protein in Ang Ⅱ Induced H9C2 Cell Hypertrophy Model

作　　者：任晓萌[1,2] 陈钰[3] 李洁[2] 吴丽君[1] 杨欣宇[1,2] 吴爱明[1] 邢雁伟[2] 商洪才[1] 高永红[1] 

机构地区：[1]北京中医药大学东直门医院中医内科学教育部重点实验室和北京市重点实验室,北京100700 [2]中国中医科学院广安门医院 [3]福建卫生职业技术学院

出　　处：《中西医结合心脑血管病杂志》2016年第22期2609-2612,共4页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：国家自然基金(No.81373935,81430098);北京中医药大学校级杰青项目(No.2015-JYB-XJQ001)

摘　　要：目的研究稳心颗粒(WXKL)对血管紧张素Ⅱ(AngⅡ)诱导H9C2细胞肥大模型骨架蛋白的影响。方法体外培养H9 C2细胞,经血管紧张素Ⅱ诱导复制心肌细胞肥大模型,加入5 g/L稳心颗粒后,观察细胞的形态变化以及对骨架蛋白的影响。结果经AngⅡ诱导的H9C2心肌细胞形态发生改变,细胞横向拉伸,肿胀变大,细胞体积较正常心肌细胞增大;加入稳心颗粒后H9 C2心肌细胞形态有所恢复,但还是比正常细胞横向宽度增加。应用激光共聚焦显微镜下观察细胞形态发现,正常H9 C2细胞经10-7mol/L AngⅡ刺激48 h后,细胞形态明显肿胀变大,细胞的长度和宽度分别由(120.32±8.36)μm和(51.82±8.62)μm增加到(182.67±7.81)μm和(92.22±7.56)μm。加入稳心颗粒作用24 h后,AngⅡ组的H9C2心肌细胞形态有所恢复,细胞的长度和宽度为(160.35±5.66)μm、(55.87±4.52)μm。结论稳心颗粒在AngⅡ诱导的肥厚心肌H9C2细胞模型中,能改善细胞骨架蛋白,起到一定的改善心肌肥厚的作用。Objective To study the effect of Wenxin granule（ WXG） on the cytoskeleton protein of angiotensin Ⅱ（ AngⅡ） induced H9 C2 cell hypertrophy model. Methods H9 C2 cells were cultured in vitro,the model of cardiomyocyte hypertrophy was induced by AngⅡ. After dosing WXG with 5 g / L,the morphological changes of the cells and the cytoskeleton were observe. Results The morphological of H9 C2 cell induced by AngⅡ had changes,cell transverse tensile,swelling becomes larger,cell volume vs normal cells. After with the addition of WXG,H9 C2 myocardial cell morphology had been restored,but still larger than the transverse width of normal cells. And the discovery of cell morphology were observed under confocal laser scanning microscopy（ CLSM）,cell morphology was apparent swelling becomes larger,the length and the width of the cell respectively by 120. 32 μm ± 8. 36 μm and 51. 82 μm ± 8. 62 μm increased to 182. 67 μm ± 7. 81 μm and 92.22 μm ± 7. 56 μm. After 24 hours of WXG,H9 C2 myocardial cell morphology in AngⅡ group had been restored,the length and the width of the cell to 160. 35 μm ± 5. 66 μm,55. 87 μm ± 4. 52 μm. Conclusion In H9 C2 cell model of hypertrophic myocardium induced by AngⅡ,the WXKL can improve the cytoskeletal protein,which plays a role in improving cardiac hypertrophy.

关 键 词：心肌肥厚 H9C2心肌细胞 血管紧张素Ⅱ 稳心颗粒 

分 类 号：R285[医药卫生—中药学]