淫羊藿苷对阿尔茨海默病大鼠模型COX-2表达的影响  

The Influence of Icariin on the Expression of COX-2 in Rats with Alzheimer-like Model

作　　者：甄瑾 王梅玲 朱润秀 肖梅 傅永旺 郭建雄 

机构地区：[1]内蒙古自治区人民医院,呼和浩特010017

出　　处：《中西医结合心脑血管病杂志》2016年第22期2629-2630,共2页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：内蒙古自然科学基金(No.200711020933)

摘　　要：目的观察淫羊藿苷(ICA)对脂多糖(LPS)诱导的阿尔茨海默病(AD)炎症模型大鼠海马内环氧酶-2(COX2)表达的影响。方法将36只Wistar大鼠随机分为对照组、模型组、ICA低剂量组[ICA30 mg/(kg·d)]、ICA高剂量组[ICA120 mg/(kg·d)],侧脑室注射脂多糖建立AD炎症模型(对照组注射生理盐水),连续灌胃2周,每天1次,断头取脑,免疫组化法(SP法)检测大鼠海马内COX-2蛋白的表达,用图像分析系统对染色结果进行分析;通过SYBR GREENⅠ荧光定量PCR检测大鼠海马内COX-2m RNA的表达情况。结果与模型组相比,高剂量组COX-2蛋白及m RNA的表达明显降低(P<0.01),但仍明显高于对照组(P<0.01),低剂量组COX-2蛋白及m RNA的表达与模型组比较无统计学意义(P>0.05)。结论高剂量淫羊藿苷可降低AD炎症模型大鼠海马内COX-2的表达,可抑制炎症反应对神经细胞的损伤。Objective To investigate the influence of icariin（ ICA） on the expression of cyclooxygramse 2（ COX-2） in inflammational rat model hippocampus with Alzheimer＇s disease（ AD） induced by lipopolysaccharides（ LPS）. Methods Thirty-six rats were allocated into 4 groups：control group,model group,low-dose ICA group and high-dose ICA group. The rat models of AD were induced by being injected LPS into the lateral cerebral ventricle. After two weeks of intragastric administration,brain tissue of rats was removed. The expressions of COX-2 protein and m RNA in hippocampus of the rats were detected by immunohistochemistry（ SP method） and SYBR GREEN fluorescence quantitative RTPCR. Results The expressions of COX-2 protein and m RNA in high-dose ICA group were significantly lower than those in model group（P〈 0. 01）. Conclusion The high-dose ICA can reduce the expression of COX-2 in inflammational rat model hippocampus of AD and can inhibit the inflammatory reaction of nerve cells injury.

关 键 词：阿尔茨海默病 淫羊藿苷 内环氧酶-2 脂多糖 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]