皮肤暴露邻苯二甲酸二丁酯对小鼠过敏性哮喘的影响  

Influence of dermal exposure of dibutyl phthalate on allergic asthma of mice

作　　者：李俐[1] 李金泉[2] 杨旭[2] 

机构地区：[1]湖北大学生命科学学院,武汉430062 [2]华中师范大学生命科学学院

出　　处：《公共卫生与预防医学》2016年第6期4-8,共5页Journal of Public Health and Preventive Medicine

基　　金：国家自然科学基金重点项目(51136002)

摘　　要：目的 探究皮肤暴露邻苯二甲酸二丁酯（Dibutyl phthalate,DBP）对过敏性哮喘的影响。方法 30只Balb/c雄性小鼠随机分为5组：（1）对照组;（2）Ovalbumin（OVA）致敏组;（3）4 mg/kg/d DBP＋OVA组;（4）40 mg/kg/d DBP＋OVA组;（5）400 mg/kg/d DBP＋OVA组。第1-5组小鼠每天皮肤暴露于生理盐水或不同浓度的DBP,连续28 d。第2-5组小鼠分别在实验的11、18及25 d腹腔注射OVA致敏液,并且在第29-35 d进行每日一次每次30 min的OVA（1%）雾化,构建小鼠过敏性哮喘模型。第36 d处死实验小鼠,取肺组织和血清,使用ELISA法检测肺组织中的白介素-4（interleukin-4,IL-4）、干扰素-γ（interferon-γ,IFN-γ）和血清中的免疫球蛋白E（immunoglobulin E,Ig E）含量。同时,采用HE染色法观察小鼠肺部气道的病理学变化。结果 与过敏性小鼠模型组相比,DBP皮肤暴露会提高肺组织中IL-4和血清中Ig E的含量,具有统计学意义（P〈0.01）。同时,会显著的加重小鼠肺组织的气道重塑和炎性细胞浸润等病理学变化。结论 长期皮肤暴露于DBP（〉4 mg/kg/d）会诱导小鼠产生过敏性体制,加重过敏原诱导过敏性哮喘。Objective To explore the effect of dibutyl phthalate （ DBP） on allergic asthma of mice by dermal exposure. Methods 30 male Balb/c mice were randomly divided into 5 groups ： （1 ） saline control, （2 ） OVA - sensitized group, （3 ） OVA - combined with 4 mg/kg/d DBP group, （4） OVA - combined with 40 mg/kg/d DBP group, （5） OVA - combined with 400 mg/kg/d DBP group. 28 days dermal treatments with DBP or saline were done to the mice from group 1 to 5 by the application of aqueous suspension, in which DBP was diluted in sterile saline from stock solution. The mice from group 2 to 5 were sensitized with OVA ＋ Al（ OH） 3 （5 mg OVA and 175 mg A1（0H）3 in 30 ml saline） by subcutaneous injection on the 11^th, 18^th and 25^th day. This was then followed by an aerosol challenge in 1% OVA （30 min/d） from the 29^th to 35^th day （7 times） using an ultrasonic nebulizer. All mice were sacrificed on the 36th day. The concentrations of IL - 4 and IFN - 7 in lung tissue and serum total IgE and histopathological examination were determined. Results The results of immunological and inflammatory biomarkers （ total IgE and Th cytokines） , as well as histopathological examination supported the notion that dermal exposure of DBP may promote and aggravate allergic asthma. Conclusion Dermal exposure of DBP could aggravate allergic asthma in mice.

关 键 词：邻苯二甲酸二丁酯 皮肤暴露 过敏性哮喘 过敏性炎症 

分 类 号：R256.12[医药卫生—中医内科学]