基于药效学试验的肠激安方挥发油提取工艺筛选及其GC-MS研究  

作　　者：闫雪[1] 唐洪梅[1] 李得堂[1] 何嘉仑[1] 廖小红[1] 曾德浩[1] 

机构地区：[1]广州中医药大学第一附属医院药学部,510405

出　　处：《湖南中医药大学学报》2016年第A02期1315-1315,共1页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金（81073160）;广东省自然科学基金项目（10151040701000041）;广州市科技局重大产业项目（2008A1-E4101-6）;广东省医院药学研究基金（2008A016）

摘　　要：目的 比较不同方法提取的肠激安方挥发油的镇痛、抗炎、止泻作用,从而筛选出具有优势的挥发油提取方式;并对较优方法提取的挥发油进行化学成 分分析,以 揭示其相关药效的物质基础.方法 对于采用二氧化碳超临界萃取法（SFE-CO2法）和水蒸气蒸馏 法（SD法）两种不同工艺提取的挥发 油,通过醋酸致小鼠扭体实验考察其 镇痛作用;二甲苯致小鼠耳肿考察其抗炎作用;生大黄致小鼠腹泻实验考察其止泻作用,并通过综合三个实验的结果比较其药效作用.采用 GC-MS 对较优方法提取的挥发油进行化学成分分析, 用峰面积归一法计算各化合物的相对含量.结果 在镇痛实验中,SD 法挥发油与 SEF-CO2法挥发油均能非常有效地减少小鼠的扭体次数（P〈0.01）.结论 SD 法提取的挥发油具有镇痛、抗炎、止泻作用.Objective To compare the analgesic, antiinflammatory and antidiarrheal effects of volatile oil extracted from Chang Ji An Fang by different approachs, and to extract the volatile oil from the volatile oil, and to analyze the chemical constituents of essenti al oil extracted by the optimal approach. The material basis of related efficacy. Methods The analgesic effect of volatile oil extracted by two different processes of CO2 supercritical extraction （SFE-CO2） and steam distillation （SD） was investigated by acetic acid-induced writhing test in mice. Xylene induced mice Anti-inflammatory effects of rhubarb were observed; antidiarrheal effects of rhubarb-induced diarrhea in mice were studied, and the pharmacodynamics was compared by the outcomings of three experiments. The chemical constituents of the essential oil extracted by the optimal approach were analyzed by GC-MS, and the relative contents of the compounds were calculated by the peak area normalization approach. Results In the analgesic experiment, the volatile oil of SD approach and the SEF-CO2 volatile oil were very effective in reducing the number of writhing in mice （P 〈0.01）. Conclusion SD approach of volatile oil has analgesic, anti-inflammatory, antidiarrheal effect.

关 键 词：药效学试验 肠激安方 挥发油提取工艺 GC-MS研究 

分 类 号：R2[医药卫生—中医学]