RP-HPLC法同时测定菥蓂药材中7种黄酮苷的含量  

Simultaneous determination of seven flavonoids in Thlaspi arvense L. by RP-HPLC

作　　者：程丽媛[1] 梁勇 罗轩[1] 刘旭[1] 杨华[1] 王立升[1] 

机构地区：[1]广西大学化学化工学院,广西南宁530004 [2]南宁骄阳医药科技有限公司,广西南宁530003

出　　处：《广西大学学报（自然科学版）》2016年第6期2053-2059,共7页Journal of Guangxi University（Natural Science Edition）

基　　金：广西自然科学基金重点项目(2010GXNSFD013040);广西科技攻关项目(桂科攻11107010-3-5)

摘　　要：建立了一种可以对菥蓂药材中5,7-二羟基-4＇-O-（6″-O-β-D-葡萄糖）-β-D-葡萄糖黄酮、异肥皂草苷、异荭草苷、牡荆苷、异牡荆苷、木犀草苷和大波斯菊苷的含量进行同时测定的RP-HPLC法。色谱条件：色谱柱为Agilent C18柱（4.6×150 mm,3.5μm）;流动相为乙腈-0.4%冰醋酸水溶液,梯度洗脱;流速1.0 m L/min;检测波长273 nm;柱温35℃。结果表明,在上述色谱条件下,5,7-二羟基-4＇-O-（6″-O-β-D-葡萄糖）-β-D-葡萄糖黄酮、异肥皂草苷、异荭草苷、牡荆苷、异牡荆苷、木犀草苷和大波斯菊苷的质量浓度分别在5.67-56.7μg/m L（r=0.999 7）,10.92-109.2μg/m L（r=0.999 4）,5.76-57.6μg/m L（r=0.9998）,4.92-49.2μg/m L（r=0.999 8）,13.95-139.5μg/m L（r=0.999 9）,5.25-52.5μg/m L（r=0.999 8）,5.22-52.2μg/m L（r=0.999 7）范围内与色谱峰的峰面积呈现良好的线性关系,平均加样回收率均在97%-101%,RSD均小于2.7%。该分析方法简便、快速、准确且重现性好,为有效地监控菥蓂药材的质量提供了可靠方法。A RP-HPLC method for simultaneous determination of 5,7-dihydroxy-flavone-4＇-（ 6″-O-β-D-glucopyranoside）-O-β-D-glucopyranoside,isosaponarin,isoorientin,vitexin,isovitexin,luteolin-7-O-β-D-glucoside and apigenin-7-O-β-D-glucoside in Thlaspi arvense L was established. Theseparation was performed on an Agilent C18column（ 4. 6×150 mm,3. 5 μm） with a gradient elution composed of acetonitrile and 0. 4% acetic acid aqueous solution. The column temperature was set at35 ℃,and the flow rate was 1. 0 m L / min. The detective wavelength was set at 273 nm. The results indicated that the linear range was 5. 67 - 56. 7 μg / m L（ r = 0. 999 7）,10. 92 - 109. 2 μg / m L（ r =0. 999 4）,5. 76 - 57. 6 μg / m L（ r = 0. 999 8）,4. 92 - 49. 2 μg / m L（ r = 0. 999 8）,13. 95 -139. 5 μg / m L（ r = 0. 999 9）,5. 25 - 52. 5 μg / m L（ r = 0. 999 8）,and 5. 22 - 52. 2 μg / m L（ r =0. 999 7）,respectively（ n = 6）. The average recoveries（ n = 3） of seven flavonoids were 97% -101%,and the RSDs were less than 2. 7%. The method is simple,rapid,accurate and reproducible,and can be effectively used for the quality control of Thlaspi arvense L.

关 键 词：菥蓂 RP-HPLC 黄酮苷 含量同时测定 

分 类 号：R917[医药卫生—药物分析学]