生物酶对五氯联苯降解的初步研究  

Pilot study on bioenzyme degradation to pentachlorodiphenyl

作　　者：季蕾[1] 陈贯虹[1] 傅晓文[1] 黄玉杰[1] 王加宁[1] 张强[1] JI Lei CHEN Guan-hong FU Xiao-wen HUAGN Yu- jie WANG Jia -ning ZHANG Qiang(Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Shandong Academy of Sciences, Jinan 250014, China)

机构地区：[1]山东省科学院生态研究所,山东省应用微生物重点实验室,山东济南250014

出　　处：《山东科学》2016年第6期94-97,共4页Shandong Science

基　　金：国家国际科技合作专项(2014DFE90100);山东省自然科学基金(ZR2015YL008,BS2015HZ011)

摘　　要：多氯联苯(PCBs)是具有代表性的持久性有机污染物(POPs),其氯代数目越多,降解越难。本文以分离筛选的6株PCBs降解细菌为材料,制备了含有NADH辅酶再生系统关键酶甲酸脱氢酶(FDH)的PCBs降解复合酶,研究了其对PCBs的降解效果。结果表明,Pseudomonas sp.MGB11胞内酶/FDH复合酶对五氯联苯PCB114的降解率在10 h内可达69.4%,对于高毒性、难降解的高氯PCBs的污染修复具有应用价值。Polychlorinated biphenyls （ PCBs） is a representative of persistent organic pollutants （ POPs）. More its chlorinated number leads to more difficult its degradation . We prepared PCBs compound degrading enzyme of key enzyme formate dehydrogenase （ FDH） of NADH coenzyme regeneration system with isolated 6 PCBs degradation bacteria strains as materials. We address its degrading effect to PCBs. Results indicate that degradation rate of Pseudomonas sp. MGB11/FDH compound enzyme to pentachlorodiphenyl PCB114 is up to 69.4% within 10 h. It therefore has application value in the repair for high chlorine PCBs pollutants with high toxicity and low degradability.

关 键 词：五氯联苯 降解复合酶 甲酸脱氢酶 

分 类 号：X53[环境科学与工程—环境工程]