基于EST-SSR标记的欧洲红皮柳遗传变异分析  

Analysis of genetic variation of Salix purpurea based on EST-SSR markers

作　　者：郑纪伟[1] 孙冲[1,2] 周洁 何旭东[1] 

机构地区：[1]江苏省林业科学研究院,江苏南京211153 [2]南京林业大学,江苏南京210037

出　　处：《江苏林业科技》2016年第6期6-11,37,共7页Journal of Jiangsu Forestry Science & Technology

基　　金：国家林业局948项目"高生物量灌木柳遗传资源及培育技术引进"(2012-4-38);江苏省农业科技自主创新资金项目"观赏柳树新品种选育"[CX(14)2024]

摘　　要：利用25个柳树EST-SSR标记对欧洲红皮柳18个个体进行基因分型,并通过标记多态性来检验个体间的遗传变异情况。结果表明:25个标记共检测到100条等位片段,每个位点等位基因数从1—12个不等,平均4个。其中23个标记表现为多态性,其观测杂合度(Ho)变化范围为0.055 5到1.0,平均为0.627 2;期望杂合度(He)变化范围为0.055 5到0.852 3,平均为0.567 8;多态信息量(PIC)变化范围为0.812 5到0.052 5,平均值为0.494 1。聚类分析显示,在遗传相似度为0.52处,欧洲红皮柳18个个体分为2组。该研究为欧洲红皮柳核心种质的收集与利用提供了理论依据。In this pape r ,18 individuals of Salix purpurea were genotyped by 25 EST-SSR marke rs , and the genetic variations of these individuals were evaluated through marker polymorphism. The result showed that 100 alleles had been detected from 25 EST-SSR in tota l ,of which 23 markers were polymorphic. The number of alleles ranged from 1 to 12,wdth an average of 4 alleles per locus. The observed heterozygosity （He） ,the expected heterozygosity （Ho ）and the polyinorphic inlonmtion content （PIC） varied wdth locus from 0. 055 5 to 1.0 （mean equals 0.627 2）, 0. 055 5 to 0.852 3 （mean equals 0.567 8） and 0. 052 5 to 0.812 5 （mean equals 0.494 1） respectively. Two main groups were divided with the similarity coefficient of 0.52 by dendrogram analysis. This study could provide a theoretical basis for the collection and utility of S. purpurea core germplasm.

关 键 词：欧洲红皮柳 微卫星 遗传变异 亲缘关系 

分 类 号：S792.12[农业科学—林木遗传育种] Q754[农业科学—林学]