胰岛素样生长因子1受体在甲状腺相关性眼病眼眶脂肪细胞增殖和分化中的作用  

Role of insulin-like growth factor-1 receptor in proliferation and differentiation of orbital preadipocytes in patients with thyroid-associated ophthalmopathy

作　　者：俞丹洋[1,2] 刘凤权[1] 曹芳[3] 王秋红[1] 曾程程 魏锐利[1] YU Dan-yang LIU Feng-quan CAO Fang WANG Qiu-hong ZENG Cheng-cheng WEI Rui-li(Department of Ophthalmology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China Department of Ophthalmology,Kunming General Hospital of PLA,Kunming 650032,Yunnan,China Department of Ophthalmology,Lanzhou Military Region Authorities Outpatient Department, Lanzhou 730000,Gansu, China)

机构地区：[1]第二军医大学长征医院眼科,上海200003 [2]解放军昆明总医院眼科,昆明650032 [3]兰州军区机关门诊部眼科,兰州730000

出　　处：《第二军医大学学报》2016年第12期1488-1494,共7页Academic Journal of Second Military Medical University

摘　　要：目的 观察胰岛素样生长因子1（IGF-1）受体（IGF-1R）在甲状腺相关性眼病（TAO）眼眶脂肪组织中的表达及分布情况,探讨IGF-1对眼眶前脂肪细胞增殖和分化的影响。方法 收集2014年9月至2015年1月期间入住第二军医大学长征医院的TAO组患者（n=6）和对照组患者（n=5）的眼眶脂肪组织,用蛋白质印迹法、实时定量PCR及免疫组化法检测两组患者眼眶脂肪组织中IGF-1R的表达及分布情况。分别提取两组患者的眼眶前脂肪细胞,用CCK-8法检测不同浓度（0、1、5、10、20nmol/L）IGF-1对细胞增殖能力的影响。体外分化细胞后用油红O染色法及蛋白质印迹法观察两组细胞的分化情况和IGF-1R的表达变化;实时定量PCR检测不同浓度（0、1、5、10、20nmol/L）IGF-1对TAO组细胞分化水平的影响。结果 TAO组患者眼眶脂肪组织中IGF-1R蛋白及mRNA的表达均高于对照组（P〈0.01）。不同浓度IGF-1均可以促进眼眶前脂肪细胞的增殖,且在1-20nmol/L IGF-1作用下在TAO组细胞的增殖能力均高于对照组（P〈0.05,P〈0.01）。TAO组前脂肪细胞分化前、后细胞中IGF-1R的表达水平均明显高于对照组。相较于未加IGF-1组,1-20nmol/L IGF-1均促进TAO组前脂肪细胞中PPARγmRNA的表达（P〈0.01）;油红O染色检测结果显示随着IGF-1浓度的升高,脂滴合成逐渐增加（P〈0.05,P〈0.01）。结论 IGF-1R在TAO患者脂肪组织及前脂肪细胞中的表达较高,IGF-1可以促进TAO眼眶前脂肪细胞的增殖和分化。Objective To observe the expression and distribution of insulin-like growth factor-1 receptor （IGF-1R） in orbital adipose tissues of patients with thyroid-associated ophthalmopathy （TAO）,and to explore the effect of insulin-like growth factor-1 （IGF-1） on the proliferation and differentiation of orbital preadipocytes. Methods We harvested the orbital adipose tissues from 6 patients with TAO （TAO group） and 5 controls （control group）; the patients were treated in Changzheng Hospital, Second Military Medical University from Sep. 2014 to Jan 2015. The expression and distribution of IGF-1R in the orbital adipose tissues were examined by Western blotting analysis, real-time PCR and immunohistochemistry. The orbital preadipocytes were extracted in two groups, and the effects of different concentrations of IGF-1 （0 nmol/L, 1 nmol/L, 5 nmol/L,10 nmol/L,and 20 nmol/L） on cell proliferation were examined by CCK8. The differentiation of cells and the expression of IGF-1R were observed by oil red O staining and Western blotting; the effect of different concentrations of IGF-1 （0 nmol/L,1 nmol/L,5 nmol/L,10 nmol/L,and 20 nmol/L） on preadipocyte differentiation was detected by real-time PCR. Results The expressions of IGF-1R protein and mRNA in orbital adipose tissues in TAO group were significantly higher than those in the control group （P〈0 . 01）. The proliferation of orbital preadipocytes was promoted in both groups ^ and the cell proliferation in TAO group was significantly higher than that in control group when treated with 1-20 nmol/L IGF-1 （P〈0 . 05, P〈0. 01）. The expression of IGF-1R in the TAO group was significantly higher than that in the control group before and after differentiation Compared with IGF-1 absent group,1-20 nmo l/L IGF-1 significantly promoted the expression of PPARy mRNA in orbital preadipocytes in TAO group （P〈0 . 01）. Oil red O staining results showed that the lipid droplet synthesis was significantly increased with the increase

关 键 词：GRAVES眼病 脂细胞 IGF 1型受体 胰岛素样生长因子1 细胞分化 细胞增殖 

分 类 号：R581.11[医药卫生—内分泌]