基于AgI模拟酶纳米材料构建阻抗型免疫传感器用于检测癌胚抗原  

A novel impedimetric immunosensor based on AgI mimic enzyme nanomaterial for detecting carcinoembryonic antigen

作　　者：赵燕凌[1,2] 张辉[2] 王效春[2] 谢鲜梅[1] ZHAO Yan-ling ZHANG Hui WANG Xiao-chun XIE Xian-mei(College of Chemistry and Chemical Engineering,Taiyuan University of Technology,Taiyuan 030024,Shanxi, China Department of Medical Imaging, Shanxi Medical University, Taiyuan 030001, Shanxi, China)

机构地区：[1]太原理工大学化学化工学院,太原030024 [2]山西医科大学医学影像学系,太原030001

出　　处：《第二军医大学学报》2016年第12期1533-1537,共5页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(50872086)~~

摘　　要：目的 设计一种基于AgI模拟酶纳米材料的阻抗型免疫传感器,对血清样品中的癌胚抗原（CEA）进行高灵敏检测。方法 合成一种壳聚糖修饰的碘化银（CS-AgI）纳米材料,利用透射电镜（TEM）和傅里叶红外光谱（FTIR）法对合成的CS-AgI进行表征,以CS-AgI为信号标记物修饰CEA抗体,采用夹心式免疫分析法在金电极表面制备一种高效、灵敏的电化学免疫传感器,采用电化学工作站定量分析检测所构建免疫传感器的电化学性能。结果 成功合成CS-AgI纳米颗粒,颗粒呈球形,粒径约为100nm。以CS-AgI纳米颗粒为信号标记物构建的免疫传感器在pH 7.0的磷酸盐缓冲液中检测CEA时表现出良好的电化学性能,其交流阻抗响应值与样品中的CEA浓度的对数呈线性增加趋势,并在CEA浓度为0.1-80ng/mL范围内展现出良好的线性关系（r=0.996）,检测限达到0.05ng/mL。结论 基于AgI模拟酶纳米材料构建的阻抗型免疫传感器可以高灵敏检测CEA,同时具有较好的精确性、重现性和选择性,为癌症的早期诊断和早期治疗提供了实验依据。Objective To design a novel impedimetric immunosensor based on AgI mimic enzyme nanomaterial for detecting carcinoembryonic antigen（CEA）in serum with high sensitivity.Methods A novel chitosan modified AgI（CS-AgI）nanomaterial was synthesized and was characterized by transmission electron microscope（TEM）and Fourier transform infrared spectroscopy（FTIR）.Then by utilizing CS-AgI labeled CEA antibody as tags,we prepared a novel impedimetric immunosensor on the gold electrode using the sandwich-type immunoassay.The electrochemical properties of the prepared impedimetric immunosensor were observed by electrochemical workstation and the concentration of CEA in sample was quantitatively analyzed.Results The synthesized CS-AgI nanoparticles were spherical in shape,with the particle size being 100nm;the particles were employed to construct immunosensor as signal markers.The immunosensor had an excellent electrochemical performance in detection of CEA under PBS base solution of pH=7,and its AC impedance response increased with the increase of the logarithm of CEA concentration,exhibiting agood linear relationship in the range of 0.1ng/mL to 80ng/mL（r=0.996）,with a detection limit of 0.05 ng/mL.Conclusion Based on AgI mimic enzyme nanomaterial,the impedance immunosensor prepared in this study shows a high sensitivity in detecting CEA;meanwhile,it has acceptable selectivity,repeatability and stability,providing an experimental evidence for early diagnosis and treatment of cancer.

关 键 词：生物传感技术 电化学免疫传感器 电阻抗 模拟酶 纳米粒子 壳聚糖 碘化银 癌胚抗原 

分 类 号：R446.6-3[医药卫生—诊断学]