麻醉机呼吸回路消毒方式对呼吸回路中病原菌污染的影响分析  

Influence of the respiratory circuit disinfection method of the anesthesia machine on the bacterial contamination in respiratory circuit

作　　者：刘喆[1] 周勇[1] 张军[1] 高项羽[1] 李铮[1] 李丰[1] 

机构地区：[1]南阳市中心医院麻醉科,河南南阳473000

出　　处：《中华医院感染学杂志》2016年第24期5659-5661,共3页Chinese Journal of Nosocomiology

基　　金：河南省卫生厅资助项目(201401027)

摘　　要：目的探讨麻醉机呼吸回路两种消毒方式对管路中细菌的消毒效果,为麻醉机呼吸回路消毒选择提供参考。方法 2015年1月-7月对46台封闭式回路的麻醉机分别采用传统消毒和消毒机消毒方式,在消毒前、消毒后、消毒使用7d后对内部回路系统吸气口端、呼气口端、钠石灰罐出口、入口进行采样,细菌培养检测。结果传统消毒、消毒机消毒在消毒前麻醉机回路污染率为20.29%、18.84%;消毒后未检出细菌,污染率0.00%,使用7d后污染率为2.90%、2.17%,消毒前后不同时点比较差异具有统计学意义(P<0.05);消毒前、消毒使用7d后呼气口端污染率最高,传统消毒为20.29%、2.90%,消毒机为18.84%、2.17%;消毒前标本培养出细菌137株,其中革兰阴性菌72株占52.55%、革兰阳性菌51株占37.23%、真菌14株占10.22%,消毒使用7d后标本培养出细菌13株,其中革兰阴性菌7株占53.85%、革兰阳性菌5株占38.46%、真菌1株占7.69%。结论采用传统及消毒机消毒对麻醉机呼吸回路进行消毒均能起到良好消毒作用,减少呼吸回路中细菌污染,在消毒前后回路中污染细菌中革兰阴性菌所占比例高于阳性菌。OBJECTIVE To investigate the effect of two kinds of respiratory circuit disinfection methods of the anesthesia machine on the bacterial contamination in the respiratory circuit, so as to provide a reference for the choice of the respiratory circuit disinfection methoci of the anesthesia machine. METHODS A total of 46 closed-circuit an- esthesia machines were used traditional disinfection and disinfeetor disinfection in the hospital from Jan. 2015 to Jul. 2015. The suction mouth side, exhalation side, sodium lime cans exports, and the entrance of the internal cir- cuit system were samPled, and the bacteria were cultured and detected. RESULTS The pollution rates of anesthesia machine circuit system by traditional disinfection and disinfector disinfection before disinfection were 20.29% and 18.84%, no bacterium was detected after disinfection, and the pollution rates after 7 d were 2.90% and 2.17%, the differences at the different time points were significant （P〈0.05）. The pollution rates of exhalation side were the highest before and after 7d of disinfection, which were 20.29% and 2. 90%. Totally 137 strains of bacteria were cultivated in the positive samples before disinfection, including 72 strains of gram-negative bacteria, 51 strains of gram-positive bacteria, and 14 strains of fungi, accounting for 52.55%, 37.23%, and 10.22%, respec- tively. Totally 13 strains of bacteria were cultured in positive samples after 7d of disinfection, including 7 （53.85%） strains of gram-negative bacteria, 5 （38.96%） strains of gram-positive bacteria, and 1 （7.69%） strain of fungus. CONCLUSION The use of traditional and disinfector disinfection of the respiratory circuit of the anesthesia machine can play a good disinfection effect, and reduce bacterial contamination in the respiratory circuit. Among the Contaminated bacteria, the proportion of gram-negative bacteria in the circuit before and after disinfec- tion was higher than that of gram-positive bacteria.

关 键 词：麻醉机 呼吸回路 消毒方式 病原菌 污染 

分 类 号：R378[医药卫生—病原生物学]