患儿全血淋巴细胞及血清中EB病毒核酸定量结果的比较研究  

Comparison of Epstein-Barr virus nucleic acid quantification results between lymphocytes and serum in children

作　　者：夏倩[1] 向飞艳[1] 曾叶[1] 向贇[1] 肖晗[1] 

机构地区：[1]武汉市妇女儿童医疗保健中心检验部,湖北武汉430016

出　　处：《中华医院感染学杂志》2016年第24期5704-5706,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(81201604);湖北省自然科学基金资助项目(2012FFB05301)

摘　　要：目的比较EB病毒(EBV)感染患儿全血淋巴细胞和血清中EB病毒DNA(EBV-DNA)定量分析结果的关系,探讨如何根据患儿感染状态合理选择临床标本类型。方法收集医院2015年9月-12月疑似EB病毒感染的患儿199例,分别取患儿的外周全血及血清,采用实时荧光PCR定量检测EBV-DNA及酶联免疫吸附法测定EBV-CA-IgM,数据采用SPSS 13.0软件进行统计分析。结果全血淋巴细胞EBV-DNA阳性标本95例,阳性率为47.7%;血清EBV-DNA阳性标本36例,阳性率为18.1%;血清EBV-CA-IgM抗体阳性23例,阳性率为11.6%;当血清IgM抗体阴性时,两种标本类型EBV-DNA阳性率差异有统计学意义(P<0.01);将全血及血清EBV-DNA均为阳性的标本进行配对分析,定量结果呈正相关(P<0.01)。结论全血中淋巴细胞的EBV-DNA阳性率及拷贝数均高于血清。临床上可以根据患儿的病情来选择合适的标本。OBJECTIVE To explore how to select the appropriate sample type according to the clinical condition of EBV（Epstein-Barr virus）-infected children by analyzing the relationship of results of quantitative analysis of EBVDNA between lymphocytes and serum.METHODS Peripheral blood and serum were collected from 199 pediatric patients who were selected as suspected of EBV infection patients in our hospital from Sep.to Dec.2015.EBVDNA was detected by real-time quantitative PCR and IgM was detected by enzyme-linked immunosorbent assay（ELISA）.SPSS 13.0was used for data analysis.RESULTS According to EBV-DNA,the positive cases of peripheral blood and serum in total 199 patients were 95cases（47.7%）and 36cases（18.1%）respectively.And 23 cases were EBV-CA-IgM positive,and the seroprevalence was 11.6%.When these two type specimens in IgM negative cases,there was significant difference（P〈0.01）.Meanwhile,paired-analysis of double EBV-DNA positive cases in peripheral blood and serum showed that quantitative results were positively correlated（P〈0.01）.CONCLUSIONThe positive rate and the number of copies of EBV-DNA in lymphocytes of peripheral blood were higher than serum.Select the appropriate sample according to the clinical condition of children.

关 键 词：儿童 EB病毒DNA 实时荧光定量PCR 

分 类 号：R372[医药卫生—病原生物学]