紫花苜蓿赤霉素受体基因的克隆及表达分析  

Cloning and Expression Analysis of Gibberellin Receptor Gene in Medicago sativa L.

作　　者：陈敏[1] 马琳[1] 贾聪俊[1] 刘希强[1] 龚攀[1] 王赞[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《西北植物学报》2016年第11期2159-2166,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：现代农业产业技术体系建设专项(CARS-35-1);国家国际科技合作专项(2015DFR30570);中国农业科学院科技创新工程(ASTIP-IAS10)

摘　　要：赤霉素受体(GID)是赤霉素信号转导途径的重要成员,直接影响着赤霉素对植物体效应的发挥。该研究利用同源克隆的方法,首次从紫花苜蓿中克隆得到1个赤霉素受体基因,命名为MsGID1b。序列分析发现,MsGID1b基因开放阅读框长度为1 053bp,编码350个氨基酸,推测其蛋白质分子量为39.839kD,是一个无信号肽和跨膜结构的亲水性蛋白。序列比对结果表明,MsGID1b基因与蒺藜苜蓿MtGID1b基因的核苷酸序列相似性为98%,氨基酸序列相似性为99%,且具有HSL家族典型的HGG和GXSXG保守结构域及GA、DELLA蛋白结合位点。荧光定量PCR分析表明,MsGID1b基因在紫花苜蓿各组织中的表达丰度依次为:根>盛花>初花>茎>叶>荚果;经GA3、ABA、NaCl、PEG和黑暗诱导后该基因表达上调,尤其是在GA3诱导下,MsGID1b基因的表达量一直维持在较高水平,表明MsGID1b基因可能参与紫花苜蓿的抗逆调控。Gibberellin receptor （GID） plays an important role in gibberellin signal transduction pathway, which directly affects plant growth and development. In this study, a gibberellin receptor gene, MsGID1b, was first isolated from Medicago sativa by homologous cloning. Sequence analysis showed that MsGID1b contains 350 amino acid residues with 39.839 kD molecular weight, and has high nucleotide （98%） and amino acid （99%） similarity with its orthologous gene in Medicago truncatula. Physical and chemical properties analysis defined that MsGID1b was a kind of hydrophilic protein without signal peptide and transmembrane structures. MsGID1b contains the binding site of GA, DELLA and some conserved domain of hormone sensitive lipase family （HSL） like HGG and GXSXG. Spatio and temporal expression profile investigated by real time PCR indicated that MsGID1b expressed comprehensively with its highest expression level in root. MsGID1b was sensitive to GA3, ABA, NaCl, PEG and dark treatments. It maintained a high transcript level under GA3 treatment, suggesting that MsGID1b might participate in the response of abiotic stress of M. sativa.

关 键 词：紫花苜蓿 GID1b 同源克隆 表达模式 

分 类 号：Q785[生物学—分子生物学] Q786