倍他米松ELISA检测方法的建立  

Establishment of ELISA Method for Detecting Betamethasone

作　　者：郭璐[1] 张晶[1] 沙芳芳 赵兴然 王敬[1] 王向红[1,2] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001 [2]河北省农产品加工工程技术研究中心,河北保定071001

出　　处：《动物医学进展》2016年第12期50-54,共5页Progress In Veterinary Medicine

基　　金：河北省科技计划项目(152776120D)

摘　　要：为建立倍他米松(BET)ELISA检测方法,先使BET与琥珀酸酐反应,再采用活化酯法与牛血清白蛋白(BSA)偶联制备免疫原;其次,利用免疫动物获得多克隆抗体,经Sephrose 4B-proteinA方法对抗体进行纯化;最后,建立倍他米松ELISA检测方法。结果表明,免疫抗原偶联成功,获得了亲和力较高的多克隆抗血清,间接竞争ELISA测定其滴度为102 400、IC15为6.60×10-5 ng/mL和IC50为0.97ng/mL。该方法适用于残留在动物性食品中的BET现场大批量检测。To establish betamethasone （BET） ELISA analysis method, the first step is to have BET reacted with amber acid anhydride and then coupled with bovine serum albumin （BSA） to produce immunogen with the help of active ester method; second, polyclonal antibody is obtained by immunizing rabbits, and the antibody is purified via Sephrose 4B-proteinA method; the final step is to construct BET ELISA analysis method. The results showed that immunogen succeeded in the coupling,and polyclonal antisera with high affinity were obtained. The detection by indirect competition ELISA showed its titer is 102 400, IC15 6.60 × 10^-5 ng/mL, and IC50 0.97 ng/mL. This method is applicable in mass field detection of BET residue in the animal derived food.

关 键 词：倍他米松 人工抗原 多克隆抗体 酶联免疫吸附试验 

分 类 号：S859.83[农业科学—临床兽医学]