山羊源伪狂犬病病毒的分离与鉴定  

Isolation and Identification of PRV from Goat

作　　者：朱玲云 张正学[1] 郑龙龙[1] 刘萍丹 毕峻龙 赵谦[1] 刘佳[1] 杨贵树[1] 尹革芬[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]楚雄州动物疫病预防控制中心,云南楚雄675000

出　　处：《动物医学进展》2016年第12期113-117,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金地区项目(31160509)

摘　　要：为确诊一例发病山羊是否感染伪狂犬病病毒,采集发病羊体的肺脏和脑组织进行伪狂犬病病毒gE基因PCR检测,并将病料接种至PK-15细胞分离病毒,以及进行小鼠感染试验和gD基因分析。结果表明,PCR检测结果 PRV阳性,病料接种PK-15细胞24h后,细胞开始出现细胞病变;将病毒感染小鼠,36h后小鼠出现局部奇痒、死亡;gD基因序列分析发现,分离毒株与GenBank中的PRV gD基因序列同源性均在98%以上,氨基酸同源性在99%以上;在分离毒株gD基因的808bp^837bp位置上存在缺失与变异、高变重复区。本研究成功分离获得一株羊源伪狂犬病毒,为云南省羊伪狂犬病防控和基础研究提供资料。To detect whether a goat was infected with pseudorabies, the lung and brain of the diseased sheep were collected to carry out the PCR detection of gE gene of the pseudorabies virus; and the material was inoculated into PK-15 cells to isolate the virus; as the same time,the mice infection test and gD gene analysis were made. The result of PCR showed that PRV was positive. After 24 hours of inoculation, the cells began to show cytopathic effect; the mice infected with virus showed partial itching and death after 36 hours. Furthermore, gD gene sequence analysis showed that the homology of PRV gD gene in GenBank was more than 98% and the homology of amino acid was more than 99%. The deletion and mutation of gD gene in 808 bp-837 bp locus were found in the isolates. In this study,pseudorabies virus was successfully isolated from the goat, which provided data and basic research for prevention and control of goat pseudorabies virus in Yunnan province.

关 键 词：伪狂犬病病毒 PCR鉴定 PK-15细胞 基因分析 山羊 

分 类 号：S852.659.1[农业科学—基础兽医学]