某规模化猪场伪狂犬病的诊断  

Diagnosis of Porcine Pseudorabies in a Large-scale Pig Farm

作　　者：李春禄[1,2] 马文才[1] 乔明明[2] 杨欣艳[2] 张彦明[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨陵712100 [2]北京世纪元亨动物防疫技术有限公司,北京100094

出　　处：《动物医学进展》2016年第12期118-121,共4页Progress In Veterinary Medicine

摘　　要：河南平顶山某猪场母猪出现较严重的流产和产死胎现象，且50日龄～70日龄仔猪出现神经症状，根据临床表现初步诊断为伪狂犬病。为排除猪繁殖与呼吸综合征和猪瘟，进行了实验室诊断。应用ELISA方法检测发病保育猪及母猪血清的伪狂犬病病毒野毒株gE抗体，并对发病仔猪病料进行了伪狂犬病病毒（PRV）、猪繁殖与呼吸综合征病毒（PRRsV）和猪瘟病毒（CSFV）的实时荧光定量PCR检测。结果显示，伪狂犬病病毒野毒抗体阳性，实时荧光定量PCR检测确定仔猪病料中PRV核酸阳性，PRRSV和CSFV核酸阴性。结合临床症状及实验室检测，确诊该猪场发生的是猪伪狂犬病。Some sows appear serious abortion and stillbirth in a pig farm in Pingdingshan city Henan province,and 50-70 day old piglets appear neurological symptoms. According to the clinical manifestations, the disease was primarily diagnosed as pseudorabies. The laboratory diagnosis was done in order to exclude the porcine reproductive and respiratory syndrome （PRRS） and classical swine fever （CSF）. The gE antibody in the sera to wild strain of pseudorabies virus was detected in nursery pigs and sows by ELISA,and sick piglet samples were detected for pseudorabies virus （PRV）,porcine reproductive and respiratory syndrome virus （PRRSV） and classical swine fever virus （CSFV） by real-time PCR. ELISA results showed that the pseudorabies virus antibody was positive, real-time PCR determined the pseudorabies virus nucleic acids was positive, but the nueleic acids of PRRSV and CSFV were negative in sick piglets. With a combination of clinical manifestations and laboratory tests, the definitive diagnosis was made as porcine pseudorabies in the farm.

关 键 词：猪伪狂犬病 酶联免疫吸附试验 实时荧光定量PCR 诊断 

分 类 号：S852.659.1[农业科学—基础兽医学]