近红外光谱法非破坏性测定绿豆籽粒粗蛋白质含量的研究  

Non-destructive Test of Crude Protein in Mungbean Grain by Near Infrared Reflectance Spectroscopy

作　　者：曹志敏[1] 张志肖[1] 王彦[1] 范保杰[1] 刘长友[1] 苏秋竹[1] 张丽[1] 田静[1] 

机构地区：[1]河北省农林科学院粮油作物研究所,河北石家庄050035

出　　处：《河北农业科学》2016年第5期104-108,共5页Journal of Hebei Agricultural Sciences

基　　金：国家食用豆产业技术体系项目(CARS-09-G2);国家"十二五"科技支撑计划项目(2014BAD07B05-03);河北省农业科技成果转化资金项目(2014GB2A200322);河北省科技计划项目(14226303D)

摘　　要：为了找到一种快速、简单的测定绿豆品质的方法,利用瑞典波通(Perten)公司生产的DA7200二极管阵列近红外光谱仪,以来自我国绿豆主产区的77份绿豆资源为试验材料,对样品进行光谱扫描,并测定了直链淀粉含量的参比数据。结果表明:定标集和检验集样品的蛋白质含量预测值与化学测定值之间均呈极显著的正相关,相关系数分别为0.977 2和0.963 1;所建定标模型具有较高的预测精度。本研究利用近红外光谱仪对完整绿豆粗蛋白质的分析,可直接用于育种材料选择以及突变体筛选和种质资源的评价等研究。The feasibility of measuring crude protein contents in intact seeds of mungbean by using near infrared reflectance spectroscopy （NIRS） was studied. In order to find a quick and easy method. The chemometric method of partial least square （PLS） was used. The results showed that there was a remark-able positive correlation between the predicted values and the chemical values. The correlation coefficients to chemical values in calibration set and validation set were 0.977 2 and 0.963 1,respectively. This study can be directly used for breeding material selection, mutant screening and evaluation of germplasm re-sources of research.

关 键 词：近红外光谱法 绿豆 粗蛋白质 定标 

分 类 号：S522[农业科学—作物学]