Sema4D介导的IRS-1信号通路在抑制成骨细胞分化中的作用机制研究  

Inhibition of the osteoblast differentiation by Sema4D mediated IRS-1 signaling pathway

作　　者：张韬[1] 翁艳[2] 陈冬冬[2] 周燕芸[2] 肖莉莉[2] 张怡元[2] 

机构地区：[1]厦门大学附属福州第二医院骨科研究所,福州350007 [2]厦门大学附属福州第二医院骨一区,福州350007

出　　处：《福建医药杂志》2016年第6期54-58,共5页Fujian Medical Journal

基　　金：福建省医学创新课题(2014-CXB-23);福州市科技计划项目(2015-S-141-10);福州市2015年临床重点专科建设项目(骨科)

摘　　要：目的通过siRNA干扰技术沉默轴突导向因子4D(Sema4D)基因,探讨Sema4D基因对成骨细胞分化的影响。方法采用RT-PCR、细胞免疫荧光法和Western blot测定Sema4D基因表达情况,分析沉默效率;采用ALP染色、VonKossa染色及ALP活性观测成骨细胞分化。结果 siRNA干扰技术下调了Sema4D的表达,成功构建了Sema4D基因沉默体系;Sema4DsiRNA中成骨细胞的分化明显增强,出现了一定程度的钙化。结论沉默Sema4D基因与成骨细胞的分化有关。Objective To investigate the effects on Sema4D mediated IRS-1 signaling pathway for osteoblast differentiation. Methods The RT-PCR, cell immunofluorescence and Western blot were used to detect the Sema4D expression; the ALP staining, ALP activity assay and Vonkossa staining were used to detect osteoblast differentiation. Results The Sema4D expression was decreased by the siRNA interference technology and the system of Sema4D gene silencing was success built. Conclusion Silence Sema4D gene is helpful to osteoblast differentiation.

关 键 词：siRNA干扰技术 Sema4D基因 成骨细胞分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]