新型半胱氨酸电化学生物传感器的构建及应用研究  

Determination of Cysteine Using Novel Electrochemical Biosensor

作　　者：刘继红[1] 白晓艺 邱凌航 陈晓妍[1] 王燕[1] 

机构地区：[1]山东师范大学化学化工与材料科学学院,山东济南250014

出　　处：《山东化工》2016年第23期22-24,共3页Shandong Chemical Industry

基　　金：山东师范大学国家级大学生创新创业训练计划项目研究成果(201510445110)

摘　　要：采用电化学聚合法制备了聚茜素紫/纳米金复合膜修饰玻碳电极,构建了基于导电聚合物纳米复合修饰膜的新型电化学传感器,研究了半胱氨酸在该传感器上的电化学行为,建立了测定半胱氨酸的新方法。在pH值7.4的磷酸盐缓冲溶液中,半胱氨酸在修饰电极上产生一个灵敏的氧化峰,采用计时电流法测定,线性范围为5.0×10^(-7)~7.5×10^(-5)mol/L,检出限为2.0×10^(-8)mol/L。该电化学生物传感器用于人血清样品中中半胱氨酸含量的测定,加标回收率为98.5%~101.0%,结果令人满意。A poly （Alizarin Violet ）/ Au - nanoparticles composite film modified glassy carbon electrode was prepared by electropolymeriation method. The electrochemical behavior of eysteine was studied at the prepared electrode by cyclic voltammetry, a new determination method of trace eysteine was established. The modified electrode showed an excellent electroeatalytieal effect on oxidation of eysteine. In pH 7.4 phosphate buffer solution, the biosensor showed a good linear response to cysteine over the concentration range of 5.0 × 10 -7 -7.5 × 10 -5 mol/L with a detection limit of 2.0 ×10 -s mol/L. The proposed method was applied to the determination of cysteine in human serum with recovery of 98.5 % - 101.0 %.

关 键 词：电化学传感器 半胱氨酸 茜素紫 纳米金 

分 类 号：O657.15[理学—分析化学]