右美托咪定抑制丙泊酚诱导原代培养皮层神经元凋亡  

Dexmedetomidine inhibits propofol-induced apoptosis in primary cultured cortical neurons

作　　者：李建立[1] 尹德云[2] 王蕴欣[1] 吴红海[3] 侯艳宁[3] 

机构地区：[1]河北省人民医院麻醉科,石家庄050051 [2]河北省人民医院心内科,石家庄050051 [3]白求恩国际和平医院药剂科,石家庄050082

出　　处：《重庆医学》2016年第35期4911-4913,共3页Chongqing medicine

基　　金：河北省卫生厅指令性课题资助项目(ZL20140095);2015年政府资助临床医学优秀人才培养和基础课题研究项目(361003-6)

摘　　要：目的探讨右美托咪定抑制丙泊酚诱导原代培养皮层神经元凋亡的机制。方法体外原代培养7d的大鼠皮层神经元,给予500μmol/L丙泊酚和(或)不同浓度右美托咪定处理12h后,分为丙泊酚组、右美托咪定+丙泊酚组,对照组给予同溶剂的20%脂肪乳,用四甲基噻唑蓝(MTT)法检测各组神经元存活率的变化,Hoechst33258核染色法检测神经元调亡,蛋白质印迹法(Western blotting)测定神经元磷酸化cAMP反应元件结合蛋白(pCREB)和细胞色素C(Cyt-C)蛋白水平。结果与对照组比较,丙泊酚组神经元存活率明显下降,神经元凋亡率明显增加,pCREB蛋白水平明显降低,Cyt-C蛋白水平明显增加,差异均有统计学意义(P<0.01)。与丙泊酚组比较,右美托咪定+丙泊酚组神经元存活率明显增加,神经元凋亡率明显下降,pCREB蛋白水平明显增加,Cyt-C蛋白水平明显下降,差异均有统计学意义(P<0.01)。结论右美托咪定可对抗丙泊酚引起的原代培养皮层神经元凋亡,其机制可能与增加pCREB蛋白水平,降低Cyt-C蛋白水平有关。Objective To investigate the mechanisms of the protective effects of dexmedetomidine against the propofol-in- duced neuroapoptosis in primary cultured cortical neurons. Methods The neurons were cultured for 7 days and treated with 500 μmol/L propofol and（or） different concentrations of dexmedetomidine,then were divided into the propofol treatment group,propofol＋dexmedetomidine treatment group. The neurons in the control group were treated with 20% fat emulsion dissolved in the same solvent. 12 hours after different treatments,neuron viability was measured by using MTT assay,neuroapoptosis was detected by using Hoechst33258 staining,and the levels of pCREB and Cyt-C protein were detected by using Western blotting. Results Compared with the control group,proPofol inhibited neuron viability greatly, the neuroapoptosi＇s increased greatly, the level of pCREB decreased greatly and the level of Cyt-C increased greatly, there were statistically significant differences（P〈0.01）. Compared with propofol treatment group, dexmedetomidine increased neuron viability greatly, the neuroapoptosis decreased greatly, the level of pCREB increased greatly and the level of Cyt-C decreased greatly, there were statistically significant differences. Conclusion Dexmedetomidine exerts the neuroprotective effects against propofol-induced neuroapoptosis,which may be associated with the increase of pCREB level and the decrease of Cyt-C level.

关 键 词：丙泊酚 右美托咪定 原代培养皮层神经元 凋亡 磷酸化cAMP反应元件结合蛋白 细胞色素C 

分 类 号：R614[医药卫生—麻醉学]