耐盐碱转基因玉米的获得及其抗性分析  

Production and Analysis of Saline-alkali Tolerant Transgenic Maize

作　　者：郭嘉[1,2] 孙传波[1] 杨向东[1,2] 袁英[1,2] 

机构地区：[1]吉林省农业科学院农业生物技术研究所,长春130033 [2]吉林省农业生物技术重点实验室,长春130033

出　　处：《玉米科学》2016年第6期24-29,共6页Journal of Maize Sciences

基　　金：基金项目:吉林省农业科技创新工程[吉财教(2012)1072]

摘　　要：依据Gen Bank数据库中发表的拟南芥DREB基因序列进行优化,人工合成DREB基因序列,构建植物表达载体,通过农杆菌介导法将DREB基因转入玉米自交系Hi II中,获得转DREB基因玉米材料,经过分子检测获得4个阳性转化事件。在不同浓度水平的Na Cl溶液(40、80、120 mmol/L)和Na_2CO_3溶液(10、20、30 mmol/L)胁迫下,研究其耐盐碱性。结果表明,DREB基因已经整合到玉米基因组中,转DREB基因玉米的耐盐碱性获得显著提高,80 mmol/L的Na Cl溶液和20 mmol/L Na_2CO_3溶液可以作为转DREB基因玉米的耐盐碱分析条件。Based on the published in Gen Bank database of arabidopsis thaliana DREB gene sequence optimiza-tion, synthetic DREB gene sequences, the construction of plant expression vector, the DREB gene was transferred in-to maize inbred line Hi II by Agrobacterium tumefaciens mediated transformation. Get fourpositive transformationevents. Saline-alkali tolerance of transgenic maize carrying DREB was analyzed, under different saline-stress condi-tions（Na Cl 40, 80, 120 mmol/L） and different alkali-stress conditions（Na2CO310, 20, 30 mmol/L）. The main resultswere summarized that the DREB was integrated into maize genome and the saline-alkali tolerance was improved. 80mmol/L NaCl and 20 mmol/L Na2CO3 were the appropriate concentration in saline-alkali tolerance analysis on trans-genic maize.

关 键 词：转基因玉米 NACL NA2CO3 耐盐碱 DREB基因 

分 类 号：S513.035.3[农业科学—作物学]