α-酸糖蛋白手性柱对56种手性化合物的拆分  

Enantiomeric separation of 56 racemates by HPLC usingα-acid glycoprotein chiral stationary phase

作　　者：张紫恒[1] 杨娜[1] 胡聪[1] 杜洋[1] 袁黎明[1] 

机构地区：[1]云南师范大学化学化工学院,云南昆明650500

出　　处：《化学研究》2016年第6期737-741,共5页Chemical Research

基　　金：国家自然科学基金(21275126)

摘　　要：采用α-酸糖蛋白手性固定相(Chiral-AGP),固定流动相pH、流速,化合物样品浓度,考察酸性、碱性、中性手性化合物和21个氨基酸在α-酸糖蛋白手性柱上的拆分情况.应用高效液相色谱法,α-酸糖蛋白手性柱对上述手性样品拆分,一共有8个中性化合物、3个碱性化合物、6个酸性化合物和5个氨基酸得到手性对映分离.探讨了α-酸糖蛋白手性柱对酸性、中性、碱性样品的拆分能力并进行对比,结果证明α-酸糖蛋白手性柱拆分中性化合物和酸性化合物效果最好,拆分碱性化合物和氨基酸效果不好,为α-酸糖蛋白手性柱适合于拆分哪一类型的手性化合物提供参考.Fixed pH,velocity and compounds＇ concentration of mobile phase,enantiomeric separation of acid,alkali,neutral chiral compounds and 21 amino acids was explored by HPLC using Chiral-AGP.A total of eight neutral compounds,three basic compounds,six acidic compounds and five amino acids had been enantiomeric separated applying HPLC,usingα-Acid Glycoprotein chiral stationary phase.The capability of Chiral-AGP to chiral resolution of acid,alkali,neutral chiral compounds was investigated.The results proved that the acid and neutral compounds can be used in the best resolution,however,the effect of resolution is poor for alkali and amino acids.This paper offers a reference for the apphication of chiral AGP to separate chiral compounds.

关 键 词：α-酸糖蛋白 手性拆分 液相色谱 手性化合物 

分 类 号：O658[理学—分析化学]