选育纤维素乙醇生产的高效产酶工程菌技术的研究进展  

Research Progress on Efficient Enzyme Production Engineering Bacteria Breeding Technology of Cellulosic Ethanol Production

作　　者：闫玉玲[1] 姚秀清[1] 张全[2] 曹长海[2] 

机构地区：[1]辽宁石油化工大学,辽宁抚顺113001 [2]中国石油化工股份有限公司抚顺石油化工研究院,辽宁抚顺113001

出　　处：《纤维素科学与技术》2016年第4期68-75,共8页Journal of Cellulose Science and Technology

基　　金：中国石油化工集团公司资助项目(213105)

摘　　要：综述了近年来选育高效产纤维素酶工程菌取得的最新理论研究及工艺进展,并就复合理化诱变获取高效产酶菌,以及利用原生质体融合、原生质体诱变、基因工程等技术对各种产纤维素酶、半纤维素酶菌株进行遗传改造,对纤维素酶高效产酶工程菌的选育进展进行了总结,进一步展望了选育高产纤维素酶菌株的研究方向。The latest progresses in theoretical research and technology of breeding high cellulase producing strains were summarized. Breeding technologies of efficient enzyme bacteria for the production of cellulosic ethanol were summarized, such as to obtain efficient enzyme producing bacteria by composite physical and chemical mutagenesis, and all kinds of ceUulase and hemicellulose strains could be modified by protoplast fusion, protoplast mutagenesis and gene engineering technology and so on. The prospects on breeding high cellulase producing strains in the future were presented.

关 键 词：木质纤维素 纤维素酶 菌株 原生质体 诱变 融合 基因工程 

分 类 号：Q81[生物学—生物工程] TK6[动力工程及工程热物理—生物能]