铁皮石斛茎段原球茎的诱导、分化与植株再生  

Protocorm Induction/Differentiation and Plantlet Regeneration of Stems from Dendrobium officinale Kimura et Migo

作　　者：林江波[1] 王伟英[1] 李海明[1] 吴水金[1] 邹晖[1] 李跃森[1] 戴艺民[1] 

机构地区：[1]福建省农业科学院亚热带农业研究所,福建漳州363005

出　　处：《福建农业学报》2016年第10期1075-1079,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2014R1013-3)

摘　　要：为建立铁皮石斛Dendrobium officinale茎段原球茎途径的快繁技术体系,以带腋芽的无菌茎段为材料,利用正交试验的方法,研究基本培养基、植物生长调节剂、蔗糖质量浓度和添加物对原球茎的诱导、增殖与分化和生根的影响。结果表明,原球茎诱导的最佳培养基为1/2MS+6-BA4mg·L^(-1)+NAA0.1mg·L^(-1);原球茎增殖与分化的最佳培养基分别为MS+蔗糖30g·L^(-1)和1/2MS+蔗糖10g·L^(-1)+马铃薯泥10%;生根的最佳培养基为1/2MS+NAA1mg·L^(-1)+马铃薯泥10%+香蕉泥10%,成功建立了铁皮石斛茎段从原球茎诱导到植株再生的组培技术体系。To establish an effective and rapid propagation method for Dendrobium officinale, sterile stem cuttings from the plant were used as the explants for the study. Effects of cultivation media, growth regulators, sucrose concentrations, and additives on the protocorm induction, proliferation and differentiation, as well as the rooting of the plantlets were investigated in an orthogonal experiment. The results showed that the optimal medium for the protocorm induction consisted of 1/2MS＋6-BA4 mg L-1 ＋NAA0.1 mg L-1 ; that for the proliferation, MS＋30 g L-1 ; that for the differentiation, MS＋10 g L-1 sucrose ＋10% potato slurry; and, that for the rooting, 1/ 2MS＋NAA1 mg L-1 ＋ 10% potato slurry＋ 10% banana slurry. The method appeared to enable a successful regeneration of D. officinale plantlets through tissue culture propagation.

关 键 词：铁皮石斛 原球茎 正交试验 植株再生 

分 类 号：S567.239[农业科学—中草药栽培]