低温胁迫下AMF对景天三七生长和生理特性的影响  

Effects of arbuscular mycorrhizal fungi on growth and physiological characteristics of Sedum aizoon under low temperature stress

作　　者：徐冬梅[1,2] 贺忠群[1] 赵英鹏[1] 王一鸣[1] 龙胜举 

机构地区：[1]四川农业大学园艺学院,四川成都611134 [2]绵阳农业科学研究院,四川绵阳621023

出　　处：《草业科学》2016年第12期2452-2464,共13页Pratacultural Science

基　　金：四川省教育厅项目(10ZB044)

摘　　要：以景天三七(Sedum aizoon)为试材,接种丛枝菌根真菌(AMF)后,再进行低温处理(0℃、5℃、10℃,以25℃为对照),在低温处理2、4、6、8和10d后对生长指标和相对电导率、丙二醛(MDA)含量、抗氧化酶活性、脯氨酸(Pro)含量、可溶性蛋白含量、叶绿素含量和叶绿素荧光参数进行测定,旨在探讨低温胁迫下接种AMF对景天三七生长和生理特性的影响。结果表明,低温胁迫抑制了AMF(Glomus mosseae)对景天三七根系的侵染能力和菌根的相对依赖性。与未接种对照相比,接种AMF的景天三七在株高、根长、干鲜重等方面有明显增加,0℃低温下增长效应更明显,总干重比未接种对照增加了75.38%;低温胁迫下景天三七相对电导率、MDA含量和Pro含量随胁迫温度的下降和胁迫时间的延长而增加;超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、过氧化氢酶(CAT)活性和可溶性蛋白含量则呈先上升后下降的趋势;叶绿素含量和PSⅡ原初光能转化效率(Fv/Fm)呈下降趋势。与未接种对照相比,接种AMF能显著抑制低温下景天三七细胞膜透性和MDA含量的升高,显著提高景天三七体内SOD、POD和CAT的活性,诱导产生更多的可溶性蛋白和脯氨酸;提高植株体内叶绿素含量和Fv/Fm,明显缓解低温胁迫对景天三七的伤害,增强景天三七对低温胁迫的耐受性。We used Sedum aizoon to determine the growth index,relative conductivity,malondialdehyde（MDA）content,antioxidant enzyme activity,proline content,soluble protein content,chlorophyll content,and chlorophyll fluorescence parameters following low temperature stress（0,5,10,and 25℃）for 2,4,6,8,and 10 dafter inoculation with arbuscular mycorrhizal fungi（AMF）.The results showed that mycorrhizal colonization abilities and mycorrhizal dependency were significantly（P〈0.05）inhibited by low temperature.Low temperature stress significantly inhibited the growth of S.aizoon,and the plant height,stem diameter,and root length growth were significantly greater than in the normal control.The growth effect was greater under 0℃temperature stress,and total dry weight was 75.38% greater than that of the non-AMF treatment.Under low temperature stress,relative conductivity,MDA content,and proline increased as temperature decreased and the stress time increased.Superoxide dismutase（SOD）activity,peroxidase（POD）activity,catalase（CAT）activity,and soluble protein showed a decreasing trend after an initial increase.Furthermore,chlorophyll content and Fv/Fmdeclined.Compared with the non-AMF treatment,AMF inoculation significantly inhibited the increase in relative electric conductivity and MDA content,significantly improved antioxidant enzyme activity,such as SOD,POD,and CAT,and induced greater soluble protein and proline content.It also indicated that Fv/Fmin AMF plants was significantly higher than that in the non-AMF plants.Therefore,AMF could greatly enhance the low temperature tolerance of S.aizoonand reduce damage from low temperature stress.

关 键 词：丛枝菌根真菌 景天三七 低温胁迫 生理特性 

分 类 号：S682.33[农业科学—观赏园艺]