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作 者:雷舒涵[1] 杨妮妮[1] 余倩倩[2] 张浩玮[1] 田彦锋[1] 白小明[1]
机构地区:[1]甘肃农业大学草业学院草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]华南农业大学园艺学院,广东广州510642
出 处:《草业科学》2016年第12期2475-2484,共10页Pratacultural Science
基 金:甘肃农业大学校级自列课题(GSAU-ZL-2015-054);农业部公益性行业[农业科研专项(201403048-8)]
摘 要:为分析甘肃地区10个野生观赏草种子萌发期的抗旱性,本研究设量聚乙二醇(PEG-6000)0^-1.3 MPa进行渗透胁迫,随渗透势的降低,10个野生观赏草种子的发芽率、发芽势、发芽指数、胚芽长和胚根长先增大后下降,苗鲜重逐渐下降;活力指数除狼尾草(宁县)Pennisetum alopecuroide(Ningxian)、狼尾草(灵台)P.alopecuroide(Lingtai)和知风草(灵台)Eragrostis ferruginea(Lingtai)外,其余材料呈逐渐下降的趋势。-0.1 MPa轻度胁迫促进10个野生观赏草种子的萌发,发芽率、发芽指数、发芽势、胚芽和胚根长达最大,芨芨草(兰州)Achnatherum splendens(Lanzhou)、芨芨草(武威)A.splendens(Wuwei)、知风草(灵台)、发草(天祝)Deschampsia cespitosa(Tianzhu)初始发芽天数较未胁迫对照缩短1d。≤-0.5 MPa,10个野生观赏草种子的发芽率、发芽势、发芽指数、胚芽和胚根长明显受到抑制。≤-1.3MPa,10个材料种子均未萌发。运用隶属函数法对10个野生观赏草种子萌发期抗旱性进行综合评价,强弱依次为芨芨草(兰州)>狼尾草(宁县)>芨芨草(武威)>狼尾草(灵台)>芨芨草(肃南)A.splendens(Sunan)>书带苔草(渭源)Carex rochebrunii(Weiyuan)>发草(渭源)D.cespitosa(Weiyuan)>发草(天祝)>云雾苔草(渭源)C.nubigena(Weiyuan)>知风草(灵台)。Ten wild ornamental grasses were subjected to osmotic stress of approximately 0to-1.3MPa created with polyethyleneglycol(PEG-6000).As the osmotic stress decreased,the germination rare,germination potential,germination index,plumule length,and radicle length first increased and then decreased,however,fresh weight of seedling gradually decreased;in addition to Pennisetum alopecuroide(Ningxian),Pennisetum alopecuroide(Lingtai),and Eragrostis ferruginea(Lingtai),the vigor index of other germplasm gradually decreased.Osmotic stress at-0.1 MPa promoted germination of seeds of all the ten wild ornamental grasses,their germination rate,germination potential,germination index,plumule and radicle length reached the maximum values at this osmotic stress level,and the initial germination days of Achnatherum splendens(Lanzhou),Achnatherum splendens(Wuwei),Eragrostis ferruginea(Lingtai),Deschampsia cespitosa(Tianzhu)were one day shorter than the control.Beyond-0.5MPa,the germination of these ten wild ornamental grass seeds was severely suppressed.Furthermore,beyond-1.3MPa,seeds of all these varieties did not germinate at all.Using the subordinate function analysis to evaluate drought resistance,the varieties were ranked as follows:Achnatherum splendens(Lanzhou)〉Pennisetum alopecuroide(Ningxian)〉Achnatherum splendens(Wuwei)〉Pennisetum alopecuroide(Lingtai)〉Achnatherum splendens(Sunan)〉Carex rochebrunii(Weiyuan)〉Deschampsia cespitosa(Weiyuan)〉Deschampsia cespitosa(Tianzhu)〉Carex nubigena(Weiyuan)〉Eragrostis ferruginea(Lingtai).
关 键 词:PEG-6000胁迫 野生观赏草 种子萌发 抗旱性
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