基于元基因组的南海海绵Theonella swinhoei共生微生物次级代谢潜力研究  

The secondary metabolism potential of microbial consortia of the South China sea sponge Theonella swinhoei revealed by metagenomics

作　　者：柴光俊 李金龙[1] 冯国芳[1] 刘放[1] 孙伟[1] 张风丽[1] 李志勇[1] 

机构地区：[1]上海交通大学微生物代谢国家重点实验室,生命科学技术学院,上海200240

出　　处：《中国海洋药物》2016年第6期15-23,共9页Chinese Journal of Marine Drugs

基　　金：国家高技术研究发展计划项目(2013AA092901);国家自然科学基金项目(41176127 31300104)资助

摘　　要：目的揭示中国南海海绵Theonella swinhoei共生微生物次级代谢潜力。方法用Illumina Hiseq 2000测序仪对T.swinhoei元基因组DNA进行测序,用MG-RAST平台对共生微生物种群结构和功能进行分析,并比较此海绵与其他海绵的次级代谢差异。用fragment recruitment方法评估T.swinhoei元基因组中具有代表性的微生物在此种海绵和其他生境中的丰度。用antiSMASH预测T.swinhoei元基因组中次级代谢产物生物合成基因簇,并通过序列比对分析其来源。结果T.swinhoei共生微生物主要包括Proteobacteria(30.5%)、Actinobacteria(6.2%)、Poribacteria(3.6%)、Firmicutes(3.5%)、Chloroflexi(2.6%)和Cyanobacteria(2.1%)。Candidatus Entotheonellasp.TSY1和Candidatus Entotheonellasp.TSY2在T.swinhoei中的丰度比其他生境高很多。T.swinhoei共生微生物中聚酮合酶模块和相关蛋白(COG3321)以及非核糖体肽合成酶模块和相关蛋白(COG1020)基因的比例比其他海绵共生微生物中的比例高。次级代谢产物生物合成基因簇除来自Candidatus Entotheonella外,还有许多来自未知的共生细菌和可能的蓝细菌。结论中国南海海绵T.swinhoei共生微生物具有很强的次级代谢潜力,为研究海绵天然产物微生物来源提供了依据,同时为进一步开发利用T.swinhoei共生微生物资源提供了基础。Objective To explore the secondary metabolic potential of the microbiota of sponge Theonella swinhoei from the South China Sea.Methods T.swinhoei metagenomic DNA was sequenced by Illumina Hiseq 2000 platform.Taxonomic assignment and functional categories of reads were performed by the MG-RAST sever.Comparisons of secondary metabolism differences between this sponge and others were made based on COG database.Fragment recruitment analysis was used to estimate the dataset＇s representative microbes,and compare the differences in abundance of the representative microbes between this sponge and other habitats.Secondary metabolite biosynthesis gene clusters were predicted by antiSMASH,and the sources of these clusters were analyzed using BLAST.Results The abundant bacterial phyla in T.swinhoei metagenome were Proteobacteria（30.5%）,Actinobacteria（6.2%）,Poribacteria（3.6%）,Firmicutes（3.5%）,Chloroflexi（2.6%）and Cyanobacteria（2.1%）.The abundances of Candidatus Entotheonella sp.TSY1 and Candidatus Entotheonella sp.TSY2 in T.swinhoei were higher than those in other habitats.The metagenomic read fractions of polyketide synthase modules and related proteins（COG3321）and non-ribosomal peptide synthetase modules and related proteins（COG1020）in this sponge microbiota were higher than those in the microbiota derived from other sponges.Besides Candidatus Entotheonella,secondary metabolite biosynthesis gene clusters of the sponge microbiota were associated with unknown bacteria and putative cyanobacteria.Conclusion The results showed that the microbiota in sponge T.swinhoei from the South China sea had great secondary metabolic potential,and provided evidence for microbial origin of sponge-derived compounds,which laid a basis for further utilization of T.swinhoei symbiotic microbes in the production of marine natural products.

关 键 词：Theonella swinhoei 元基因组 海绵共生微生物 次级代谢潜力 

分 类 号：R931[医药卫生—生药学]