1种多棘海盘车内脏多糖的提取分离和结构表征  

Extraction,separation and structural characterization of polysaccharides from Asteriasamurensis viscera

作　　者：崔文聪[1] 宋博[1] 单鑫迪[1] 丛大鹏[1] 于广利[1] 赵峡[1] 

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,山东省糖科学与糖工程重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2016年第6期24-30,共7页Chinese Journal of Marine Drugs

基　　金：国家基金委山东联合基金项目(U1406402);国家科技支撑计划项目(2013BAB10B02)资助

摘　　要：目的从多棘海盘车(Asterias amurensis)内脏中提取、分离纯化多糖,并对其基本理化性质和结构进行表征。方法采用沸水提取与酶解联用的方法从多棘海盘车内脏脱脂粉中提取粗多糖;采用Q Sepharose FF强阴离子交换和Sephacryl S-100凝胶渗透色谱对粗多糖进行分离纯化;采用硫酸-苯酚法、Folin-酚法、高效凝胶渗透色谱-十八角激光散射仪(HPGPC-MALLS)和高效液相色谱(HPLC)分别进行总糖含量,蛋白含量,分子量和单糖组成进行分析;采用甲基化、红外光谱(IR)、气质联用(GC/MS)和一维、二维核磁共振波谱(NMR)法对纯化多糖结构进行表征。结果从多棘海盘车内脏中提取粗多糖的收率为3.3%。经过离子交换和分子排阻色谱分离纯化,得到1种分子量均一的多糖组分F2-A,该多糖主要由葡萄糖(Glc)组成,其糖含量为97.6%,分子量为270 8kDa,是一种以α-(1(4)-Glc为主链并含有少量β-(1(3,4)分支的海星内脏来源葡聚糖。结论从多棘海盘车内脏中纯化得到1种高分子量、结构独特的葡聚糖,为将来从事其结构和生物活性关系的研究提供了有用信息。Objective To extract and purify polysaccharides fromAsterias amurensis viscera,and investigate their chemical properties and structural features.Methods The polysaccharide was extracted by hot water combined with enzymolysis from the delipid powder of Asterias amurensis viscera.Then the polysaccharide was isolated and purified by columns of Q Sepharose FF strong anion exchange chromatography and Sephacryl S-100 gel permeation chromatography.The contents of total sugar,protein,molecular mass,and monosaccharide composition were analyzed by sulfuric acid-phenol,Folin-phenol,high performance gel permeation chromatography-eighteen multi-angle laser light scatter（HPGPCMALLS）,and high performance liquid chromatography（HPLC）,respectively.The structural characterization of the purified polysaccharide was confirmed by methylation,infrared spectroscopy（IR）,gas chromatography-mass spectroscopy（GC/MS）and nuclear magnetic resonance spectroscopy（NMR）methods.Results The total yield of the crude polysaccharide was 3.3%.A polysaccharide F2-A with uniform size and charge was obtained by ion exchange and gel exclusion chromatographic separation.The purified polysaccharide was mainly composed of glucose（Glc）,and the total sugar content was 97.6%,the molecular mass was 2708 kDa.It is a kind of glucan whose backbone wasα-（1（4）-Glc withβ-（1（3,4）branch.Conclusion A glucan withα-（1（4）-Glc backbone andβ-（1（3,4）branch was purified fromAsteria samurensis viscera.These data provided useful message for its structure-biological activity relationship study in the future.

关 键 词：多棘海盘车 内脏多糖复合物 提取 分离 结构表征 

分 类 号：R931.74[医药卫生—生药学]