人脂肪来源间充质干细胞库的初步建立  

Incipient establishment of human adipose-derived mesenchymal stem cells bank

作　　者：徐潇[1] 张旭毅[2] 闫俊灵[1] 陈冲[1] 赵欣[1] 李明辉[1] 汤苏阳[1] 

机构地区：[1]武警总医院皮肤再生医学科,北京100039 [2]武警总医院科训科,北京100039

出　　处：《解放军医学杂志》2016年第12期992-997,共6页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨建立生物学性状稳定的人脂肪来源间充质干细胞(h ADSCs)库的可行性,旨在为组织工程种子细胞提供更多来源。方法对分离得到的h ADSCs采用液氮低温冻存,并在一定时间复苏培养,以流式细胞仪检测其表面抗原,在诱导培养基中对其进行成脂和成骨诱导培养,光镜下观察细胞形态,免疫组织化学方法检测成骨诱导的特异标志物碱性磷酸酶(ALP)的表达,观察h ADSCs在复苏前后的分化能力。结果从脂肪组织中分离培养扩增获得数目稳定的h ADSCs,经冻存并复苏后的h ADSCs形态和表面抗原保持不变,可继续扩增10代以上,倍增时间为48h。复苏后第2、第6、第10代h ADSCs均保持了较强的成脂及成骨分化能力。结论初步建立h ADSCs库,可为再生医学修复重建组织工程提供较好的种子细胞。Objective To explore the possibility of establishing the human adipose-derived mesenchymal stem cells （hADSCs） bank as to provide an alternative source for the seed cells of tissue engineering. Methods The cell surface antigens of the purified, expanded hADSCs and the ones following cryopreservation were detected by flow cytometry, cultured in an induced culture medium to induce the osteogenic and adipogenic differentiation. The specific marker alkaline phosphatase （ALP） in osteogenic special medium was also detected by immunohistochemistry. Results The phenotype and expansion possibility of hADSCs after cryopreservation were remained. It could expand for 10 generations. The doubling time was 48 hours. The 2nd, 6th and 10th generation of hADSCs kept stronger ability of osteogenic and adipogenic differentiation. Conclusion The bank of hADSCs has been incipiently established and can provide eligible seed cells for tissue engineering.

关 键 词：脂肪来源间质干细胞 干细胞库 低温保存 可行性研究 

分 类 号：R329.29[医药卫生—人体解剖和组织胚胎学]