晚期大肠癌患者中医证素与KRAS、BRAF、NRAS基因突变情况的相关性研究  

Correlation Research between Syndrome Elements of Chinese Medicine and KRAS,BRAF,NRAS Gene Mutations in Advanced Colorectal Cancer Patients

作　　者：许仲宁[1,2] 杨宇飞[1] 

机构地区：[1]中国中医科学院西苑医院肿瘤科,北京100091 [2]四川省肿瘤医院中西医结合科,成都610041

出　　处：《中国中西医结合杂志》2016年第12期1445-1448,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：中国中医科学院基本科研业务费自主选题资助项目(No.ZZ070854);北京市科技计划项目(No.Z131107002213101)

摘　　要：目的研究晚期大肠癌患者KRAS、BRAF、NRAS基因突变情况与中医证素的相关性。方法收集33例晚期大肠癌患者中医证素和KRAS、BRAF、NRAS基因情况(突变"+"或野生非突变"-")。采用二分类Logistic回归分析基因突变情况与证素的相关性。结果 KRAS野生型与肝郁呈负相关(P=0.043,P<0.05),KRAS突变型与肝郁呈正相关(P=0.043,P<0.05)。KRAS野生型、KRAS突变型均与脾虚、肺虚、肾虚、阳虚、阴虚、气虚、血虚、痰浊、气滞、血瘀相关性不显著(P>0.05)。BRAF野生型、NRAS野生型均与肝郁、脾虚、肺虚、肾虚、阳虚、阴虚、气虚、血虚、痰浊、气滞、血瘀逻辑相关性不显著(P>0.05)。BRAF突变型、NRAS突变型检测阳性者少,未纳入统计分析。结论晚期大肠癌患者KRAS基因突变情况与肝郁存在相关性。KRAS野生型与肝郁呈显著负相关,KRAS突变型与肝郁呈正相关,BRAF、NRAS突变与否与中医证素无直接相关性。Objective To study the correlation between syndrome elements of Chinese medicine（CM）and KRAS,BRAF,NRAS gene mutations in advanced colorectal cancer patients.Methods Syndrome elements of CM,KRAS,BRAF,NRAS gene mutations（mutation ＂ ＋＂ wild type/non-mutation ＂-＂）were collected in33 advanced colorectal cancer patients.The correlation between gene mutations and syndrome elements of CM was analyzed using binary Logistic regression.Results Wild type KRAS（KRAS-）was negatively correlated with Gan depression syndrome（P=0.043,P〈0.05）,while KRAS mutation（KRAS＋）was positively correlated with Gan depression syndrome（P=0.043,P〈0.05）.KRAS-and KRAS＋ had no significant relation with Pi deficiency,Fei deficiency,Shen deficiency,yang deficiency,yin deficiency,qi deficiency,blood deficiency,phlegm turbidity,qi stagnation,and blood stasis（P〈0.05）.BRAF-and NRAS-had no significant relation with Gan stagnation,Pi deficiency,Fei deficiency,Shen deficiency,yang deficiency,yin deficiency,qi deficiency,blood deficiency,phlegm turbidity,qi stagnation,and blood stasis（P〉0.05）.Positive BRAF and NRAS were seldom found in this study,so they were not statistically analyzed.Conclusions There existed correlations between gene mutations and Gan stagnation.KRAS-was negatively correlated with Gan depression,while KRAS＋ was positively correlated with Gan depression.Whether there exists mutation of BRAF or NRAS was not correlated with syndrome elements of CM.

关 键 词：大肠癌 中医证素 肿瘤基因突变 

分 类 号：R273[医药卫生—中西医结合]