FOXM1在食管鳞癌中的表达及对其放射敏感性的影响  

Expression of FOXM1 and its effects on radiation sensitivity in esophageal squamous cell carcinoma(ESCC)

作　　者：刘琅嬛 罗爱武[1] 陈科良[1] 谷蒙蒙[2] 肖倍倍 邹士涛[3] 何超[3] 周俊东[3] 黄波[1] 

机构地区：[1]南华大学公共卫生学院,衡阳421001 [2]苏州大学放射医学与防护学院,苏州215123 [3]南京医科大学附属苏州市立医院,南京215001

出　　处：《辐射研究与辐射工艺学报》2016年第6期21-28,共8页Journal of Radiation Research and Radiation Processing

基　　金：湖南省自然科学基金项目(2016JJ2115);南华大学博士启动基金(2015XQD31);湖南省卫生计生委科研计划课题项目(C2015-17)资助~~

摘　　要：采用Real-time PCR检测40例食管鳞状细胞癌及相对应癌旁组织中FOXM1(Forkhead box M1)m RNA表达情况;免疫组织化学方法检测94例食管鳞状细胞癌组织中FOXM1蛋白的表达情况,分析其与鳞状细胞癌临床病理之间的关系;采用针对FOXM1的si RNA沉默ECA-109、TE-1细胞中FOXM1基因表达水平,通过免疫荧光染色、克隆形成实验检测FOXM1对食管鳞状细胞癌放射敏感性的影响。结果表明,FOXM1m RNA表达水平在食管癌组织中显著高于癌旁组织(p<0.01),其蛋白表达水平与病人生存期负相关。si RNA能有效干扰ECA-109细胞中的FOXM1表达水平,FOXM1沉默细胞中X-射线诱发的γH2AX焦点显著多于对照细胞,FOXM1沉默细胞电离辐射后克隆形成能力显著低于对照细胞。结果提示,FOXM1在食管鳞癌中的表达增加,其表达水平与病人预后负相关;FOXM1基因沉默可显著增强细胞放射敏感性,因此,FOXM1有可能成为食管鳞癌的治疗靶点。Real-time PCR was used to examine the expression of FOXM1 （Forkhead box M1） in 40 esophageal squamous cell carcinoma （ESCC） tissues, and the adjacent normal tissues. Immunohistochemistry was used toexamine the expression of FOXMI protein in 94 ESCC tissues, and to analyze the relationship between ESCC and clinic pathologic. A small interfering RNA targeting FOXM1 was designed to silence the endogenous FOXM1 expression of ECA-109 and TE-1 cells. The effect of FOXM 1 on radiation sensitive in esophageal squamous cell was observed by immunofluorescence stain, and colony formation assay. The results showed that FOXM1 mRNA expression level in esophageal cancer tissues was significantly higher than that of the adjacent tissues （p〈0.01）, and the protein level expression of it has negative correlation with the survival times; the designed siRNA could dramatically silence FOXM1 expression in ECA-109 cells; yH2AX focus induced by X-rays in FOXM1 silent cells was significantly higher than that in control cells; the clone formation ability of the silent FOXM1 group was significantly lower than that in the control group. The content of FOXM1 is improved in ESCC, and is negatively correlated with the prognosis of patients; the cell radiation sensitive proliferation is significantly improved in the silent FOXM1 ECA-10^9 cells. In conclusion, FOXM1 has the potential to be the treatment of esophageal squamous carcinoma target.

关 键 词：食管鳞癌 FOXM1不良预后 辐射敏感性 DNA损伤修复 

分 类 号：TL71[核科学技术—辐射防护及环境保护]