EMP1基因对口腔鳞癌细胞增殖、迁移和侵袭影响的体外研究  

Effects of EMP1 on the Proliferation,Migration and Invasion of Oral Squamous Cancer Cells in Vitro

作　　者：张军[1] 代晓华[2] 邹慧儒[2] 连小丽[2] 李燕妮[2] 

机构地区：[1]天津市口腔医院口腔颌面外科,天津300041 [2]天津市口腔医院中心实验室,天津300041

出　　处：《口腔医学研究》2016年第12期1240-1243,共4页Journal of Oral Science Research

基　　金：天津市卫计委科技基金项目(编号:2013KZ053)

摘　　要：目的:体外观察EMP1基因对口腔鳞癌细胞增殖、迁移和侵袭的影响。方法:构建pEGFP-N1-EMP1表达载体,稳定转染口腔鳞癌细胞系Tb3.1,并以野生型Tb3.1和pEGFP-N1稳转的Tb3.1为对照,用MTT检测细胞增殖,绘制细胞生长曲线;流式细胞仪检测细胞周期变化;用Transwell小室检测细胞的迁移和侵袭能力;组间比较采用单因素方差分析。结果:相比野生型Tb3.1和Tb3.1-pEGFP-N1细胞,Tb3.1-pEGFP-N1-EMP1细胞生长受到抑制,同时其S+G2-M期细胞比例减少(P<0.05),迁移和侵袭细胞数目减少(P<0.05)。结论:EMP1在口腔鳞癌的发生、发展中作为抑癌基因可能对口腔鳞癌细胞的增殖、迁移和侵袭起抑制作用。Objective：To investigate the effects of EMP1 on the proliferation and invasion of oral squamous cancer cells（OSCC）in vitro.Methods：pEGFP-N1-EMP1 expression vectors were constructed and transfected stably into Tb3.1cell.Tb3.1-pEGFP-N1-EMP1 cell,wild type Tb3.1cell and Tb3.1-pEGFP-N1 cell were tested using MTT to assay cell proliferation.Cell cycle and invasion were analyzed by flow cytometry and invasion assay,respectively.One-way ANOVA was adopted to compare among groups.Results：Compared with wild type Tb3.1cell and Tb3.1-pEGFP-N1 cell,the growth of Tb3.1-pEGFP-N1-EMP1 cell was inhibited,and the proportion of cells in S and G2-M phase and the number of invasive cells were reduced in Tb3.1-pEGFP-N1-EMP1cell（P〈0.05）.Conclusion：EMP1may negatively regulate the proliferation,migration and invasion of tumor cells in the oncogenesis and development of OSCC.

关 键 词：EMP1基因 口腔鳞癌细胞 转染 增殖 迁移 侵袭 

分 类 号：R739.85[医药卫生—肿瘤]