pH控制下果蔬垃圾酸化反应器中微生物优势菌群识别  

Identification dominant bacteria in acidification bioreactor fed by fruit and vegetable waste under pH control

作　　者：马海玲[1] 吴远远[1] 郑明月[1] 徐恒[1] 汪翠萍[1] 王凯军[1] 

机构地区：[1]清华大学环境学院环境模拟与污染控制国家重点联合实验室,北京100084

出　　处：《环境工程学报》2016年第12期7349-7354,共6页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(21206084);国家水体污染控制与治理科技重大专项(2012ZX07205-002)

摘　　要：以污水处理厂厌氧消化池污泥作为接种物,考察中温,pH为4.0±1,有机负荷13 g·(L·d)^(-1),水力停留时间3 d的酸化反应器处理果蔬垃圾的运行情况,并通过16S rRNA高通量测序技术及厌氧滚管分离技术识别反应器内优势菌群。结果表明,在pH为4的全混流反应器(CSTR)运行过程中,发酵产物以乳酸为主。反应器稳定运行期,乳酸杆菌属(Lactobacillus)的相对丰度达81.9%,为反应器中优势菌属。经厌氧滚管技术分离筛选获得的菌株EL1为乳酸杆菌,其发酵类型为异型发酵。A continuous stirred-tank reactor（ CSTR） with pH control（ 4. 0 ± 1） at an organic loading rate was kept at 13 g·（ L·d）^-1. The hydraulic retention time was 3 days and was setup by feeding fruit and vegetable waste under a temperature of 35 ℃. Molecular biological technology,including high throughput pyrosequenching of 16 S rRNA gene and the anaerobic roll-tube method was utilized to investigate the overall microbial community structure and the dominant bacteria. Diversity analysis using 16 S rRNA indicated Lactobacillus was dominant（ 81. 9%） followed by Aeriscardovia（ 9. 5%） at steady operational conditions. Furthermore,the strain of Lactobacillus initially called EL1 was screened,which exhibited an efficient production of lactic acid.

关 键 词：酸化反应器 PH 果蔬垃圾 乳酸杆菌 

分 类 号：X703[环境科学与工程—环境工程]