乌司他丁对光损伤大鼠视网膜的保护作用  

Protective effect of ulinastatin on light-induced retinal injury in rats

作　　者：刘科峰[1] 黄海玲[2] 朱明琼[3] 黄艳琼[3] 陈晓岚[1] 彭忠禄[4] 邝国平[3] 

机构地区：[1]湘南学院基础医学院,湖南郴州423000 [2]河北联合大学附属医院护理部,河北唐山063000 [3]郴州市第一人民医院眼科,湖南郴州423000 [4]湘南学院药学院,湖南郴州423000

出　　处：《中国临床研究》2016年第12期1601-1605,共5页Chinese Journal of Clinical Research

基　　金：湖南省教育厅资助科研项目(12C0893);湖南省重点建设学科资金资助[湘教发(2011)76号];湖南省高校重点实验室资金资助[湘教通(2008)246号]

摘　　要：目的 探讨乌司他丁对大鼠视网膜紫外线光损伤的保护作用及其机制。方法 将36只SD大鼠随机分为对照组、光损伤组和给药组,每组各12只计24只眼。采用光照强度为（2 200±138）Lux的300~400 nm紫外线间歇照射法制作视网膜损伤模型。光照前3 d起,给药组腹腔单剂量注射乌司他丁2×104U·kg-1·d-1,其他组给予等量生理盐水腹腔注射至取标本日。分别于光损伤后3 d、7 d处死大鼠,摘出眼球。HE染色后观察大鼠视网膜的组织学变化,免疫组织化学方法检测B细胞淋巴瘤/白血病-2蛋白（Bcl-2）和Bcl-2相关X蛋白（Bax）在视网膜的相对表达量。结果 对照组大鼠视网膜结构完整,层次清楚;光损伤组的视网膜结构紊乱,且随时间延长紊乱程度明显加重;给药组大鼠视网膜形态较对照组稍微紊乱,但紊乱程度均轻于相同时间段光损伤组。光损伤组大鼠的视网膜外核层（outer nuclear layer,ONL）厚度均低于同时间段对照组（P〈0.05,P〈0.01）;给药组大鼠的各时间段视网膜ONL厚度均高于同时间段光损伤组（P〈0.05,P〈0.01）,但低于正常对照组（P均〈0.01）。光损伤组中Bax蛋白在视网膜的相对表达量明显高于对照组,给药组中,Bax在视网膜的表达也较对照组明显增强,但明显弱于相应时间段光损伤组（P均〈0.05）。光损伤组的Bcl-2蛋白在视网膜的相对表达量明显低于对照组（P〈0.05）,给药组Bcl-2蛋白相对表达量较对照组明显降低（P〈0.05）,但较相应时间段光损伤组的相对表达量明显增加（P〈0.05）。结论 乌司他丁对紫外线照射造成的视网膜损伤具有保护作用,其机制可能与乌司他丁抑制Bax蛋白表达而促进Bcl-2蛋白表达从而抑制紫外线诱导的凋亡有关。Objective To explore the protective effect of ulinastatin on ultraviolet（UV） light damage on rats retina and its mechanism. Methods Thirty-six SD rats were randomly divided into control group, light damage group and medication group （n = 12 each,a total of 24 eyes）. The retinal damage models in rats were established by intermittent illumination of 300 -400 nm UV with light intensity（2 200 ±138）Lux. Intraperitoneal injection of ulinastatin of a single dose （ 2 ×104 U· kg-1· d- l ） was given in medication group from the third day before illumination until the date of taking samples, and same amount of normal saline was given in other two groups at the same time. All rats were killed at the 3-,7-day after light damage,respectively,and the eyeballs were picked out. The histological changes of the retina after HE staining were ob- served,and relative expression quantities of Bax and Bcl-2 proteins in the retina were detected by immunohistochemistry. Results In control group, the retina had complete structure and clear layer. In light injury group , the rat retinal structure disordered, and the degree of disorder was obviously aggravated with the extension of time. In medication group, rat retinal morphology slightly disordered compared with control group, but the degree of disorder was milder than those in light injury group at corresponding time points. The rat retinal outer nuclear layer （ONL） thicknesses at different time points in light injury group were significantly lower than those in cntrol group at corresponding time points （P 〈 0.05, P 〈 0.01 ）. The rat retinal ONL thicknesses at different time points in medication group were significantly higher than those in light injury group （P 〈 0.05, P 〈0. 01 ）, but significantly lower than those in control group at corresponding time points （all P 〈 0.0O1 ）. The relative expression level of Bax protein in retina of light injury group increased significantly compared with control group, and it in medication group increased

关 键 词：视网膜光损伤 乌司他丁 视网膜外核层 B细胞淋巴瘤/白血病-2蛋白 Bcl-2相关X蛋白 

分 类 号：R331[医药卫生—人体生理学]