液态羟基磷灰石胶原海藻酸钠(Alg/nHAC)人工骨促进Ilizarov骨延长骨愈合的实验研究  

The experimental study on promoting the Ilizarov distraction osteogenesis by the injectiong of liquid Alg/nHAC biocomposites

作　　者：朱超[1] 王华[1] 刘伟[1] 王邵清[1] 王斌[1] 冯庆玲[2] 刘新晖[1] 

机构地区：[1]南京医科大学附属南京江宁医院骨科,江苏南京211100 [2]清华大学材料科学与工程系,北京100081

出　　处：《生物骨科材料与临床研究》2016年第6期5-9,I0005,共6页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：江苏省自然科学基金项目BK20141436

摘　　要：目的探讨应用液态海藻酸钠/纳米羟基磷灰石/胶原(Alg/nHAC)促进肢体延长过程中骨愈合的作用。方法制备液态可注射Alg/nHAC人工骨,建立兔胫骨骨延长动物模型,随机分两组,A组,骨延长区给予微创注射Alg/nHAC人工骨,B组作为空白对照,分别在延长后2、4、8周进行组织学,X线检查、新生骨小梁百分比、骨密度以及生物力学的检测观察延长区骨愈合情况。结果延长后2、4、8周,A组在组织学、新生骨小梁百分比、骨密度以及生物力学的检测均明显优于B组,A组8周时间骨延长区已经愈合。结论液态羟基磷灰石胶原海藻酸钠(Alg/nHAC)人工骨可以通过微创注射的方式促进延长区骨愈合,为临床促进骨愈合提供了新的方法。Objective To investigate the effect of liquid sodium alginate/nanohydroxyapatite/collagen （Alg/nHAC） on promoting the healing process of bone lengthening. Methods AIg/nHAC injectable artificial bone was fabricated by using nano technology, and Animal model of rabbit tibia limbs, elongation were produced. All animals were randomly divided into two groups. After the end ofthe extension, liquid Alg/nHAC was injected into group A in the region ofelon- gation. Group B were not given any treatment as control. Histologically morphological observations, X-ray examination, biomechanical test, bone density, and biomechanical test were used to evaluate the bone healing ofextention area at 2, 4 and 8 weeks after termination of bone elongation. Results Group A were better than Group B in histologically mor- phological observations, X-ray examination, biomechanical test, bone density, and biomechanical test at 2,4 and 8 weeks after termination of bone elongation. Group A bone elongation area has healed at 8 weeks after termination of bone elongation. Conclution Liquid alginate/nanohydroxyapatite/collagen （Alg/nHAC） can promote bone healing of extended area by minimally invasive injection. It provides a new method to promote bone healing in clinical.

关 键 词：海藻酸钙/纳米羟基磷灰石/胶原 伊力扎洛夫 骨愈合 

分 类 号：R318.08[医药卫生—生物医学工程] R68[医药卫生—基础医学]